caa a22 4500 397556179 CHVBK 20180308164802.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021349 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021349 Transdominant Inhibition of Moloney Murine Leukemia Virus Proliferation by Defective Mutants of Reverse Transcriptase [Elektronische Daten] [Yukio Shirasawa, Sumie Sato, Toshio Osawa, Akikazu Hirashima] To investigate the dominant-negative inhibition of Moloney murine leukemia virus (Mo-MuLV) proliferation by polymerization-defective mutants of reverse transcriptase (RT), we constructed several plasmids harboring the Mo-MuLV RT gene in which the YVDD sequence, one of the conserved sequences in RNA-dependent polymerases, was altered, and then transformed mouse NIH3T3 cells and Escherichia coli with the mutant plasmids. Mouse NIH3T3 cells expressing these mutant RT genes were highly resistant to Mo-MuLV proliferation. The mutant RT expressed in E. coli exhibited no polymerization activity, but it retained its binding activity to the template RNA and inhibited in vitro poly(dG) synthesis occurring with the wild-type RT. These results suggest that the competition for binding of the two types of enzymes to the template is responsible for the resistance to Mo-MuLV proliferation and that the YVDD sequence of Mo-MuLV may be a good target for dominant-negative inhibition of retroviral proliferation. © by the Journal of Biochemistry Regular Papers nationallicence dominant-negative inhibition nationallicence intracellular immunization nationallicence moloney murine leukemia virus nationallicence reverse transcriptase nationallicence YXDD motif nationallicence Shirasawa Yukio Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut Sato Sumie Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut Osawa Toshio Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut Hirashima Akikazu Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1070-1075 0021-924X 119:6<1070 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021349 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021349 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shirasawa Yukio Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut NATIONALLICENCE 700 1- Sato Sumie Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut NATIONALLICENCE 700 1- Osawa Toshio Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut NATIONALLICENCE 700 1- Hirashima Akikazu Yakult Central Institute for Microbiological Research, Kunitachi, Tokyo 186 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1070-1075 0021-924X 119:6<1070 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford