caa a22 4500 397556209 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021353 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021353 Substrate Specificity of a Novel Serine Protease from Soybean [Glycine max (L.) Merrill] [Elektronische Daten] [Shimpei Morita, Masami Fukase, Masami Yamaguchi, Yuhei Morita] The substrate specificity of a novel serine protease isolated from soybean seeds, cultivar Keburi, was investigated using various peptide-MCAs and several neuropeptides involving single and paired basic ami no acid sequences. The protease was quite specific for arginine residue at the PI site of the active center, and it recognized paired Arg-Arg and cleaved at the linkage between Arg-Arg or after Arg-Arg in peptide and protein molecules. This is the first protease in plant tissues which resembles in substrate specificity the arginine-specific serine proteinases from porcine gastric and intestinal mucosa, recognizing paired basic amino acid sequences. © by the Journal of Biochemistry Regular Papers nationallicence arginine-specific protease nationallicence serine protease nationallicence soybean nationallicence substrate specificity nationallicence Morita Shimpei Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut Fukase Masami Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut Yamaguchi Masami Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut Morita Yuhei Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1094-1099 0021-924X 119:6<1094 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021353 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021353 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morita Shimpei Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Fukase Masami Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Yamaguchi Masami Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Morita Yuhei Central Research Institute, Fuji Oil Co., 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1094-1099 0021-924X 119:6<1094 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford