caa a22 4500 397556217 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021352 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021352 Situation of Monomethoxypolyethylene Glycol Covalently Attached to Lysozyme [Elektronische Daten] [Takanori So, Tadashi Ueda, Yoshito Abe, Tomoko Nakamata, Taiji Imoto] We selectively introduced monomethoxypolyethylene glycol (mPEG) 5000, 2000, and 550 into Aspll9 in lysozyme. To examine how the mPEGs were present on the surface of the modified lysozyme, the activities, binding abilities to the Fab fragment of anti-lysozyme monoclonal antibody, net charges and nuclear magnetic resonance (NMR) spectra of mPEG lysozymes were examined. With the increase in molecular weight of mPEG, the activities and binding abilities to the Fab of mPEG lysozyme decreased. However, introduced mPEGSOOO did not cause complete inhibition of the activities and binding abilities to the Fab, while the maximum length of mPEGSOOO was so great that it largely covered the surface of the lysozyme molecule. Analyses of the net charges and NMR suggested that the introduced mPEG preferentially assumed a folded conformation on the surface rather than spread all over the surface. Based on the structure of mPEG lysozyme, the mechanism of the reduced immunogenicity of mPEG lysozyme was discussed. © by the Journal of Biochemistry Regular Papers nationallicence immunogenicity nationallicence lysozyme nationallicence monomethoxypolyethylene glycol nationallicence NMR nationallicence structure ofmPEG nationallicence So Takanori Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut Ueda Tadashi Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut Abe Yoshito Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut Nakamata Tomoko Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut Imoto Taiji Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1086-1093 0021-924X 119:6<1086 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021352 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021352 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- So Takanori Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut NATIONALLICENCE 700 1- Ueda Tadashi Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut NATIONALLICENCE 700 1- Abe Yoshito Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut NATIONALLICENCE 700 1- Nakamata Tomoko Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut NATIONALLICENCE 700 1- Imoto Taiji Gradute School of Pharmaceutical Sciences, Kyushu University, Higashi-ku, 812-82 Fukuoka aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1086-1093 0021-924X 119:6<1086 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford