caa a22 4500 397556233 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021368 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021368 A New Inhibitor of Mitochondrial Fatty Acid Oxidation [Elektronische Daten] [Takashi Hashimoto, Yasuko Shindo, Masayoshi Souri, Graham S. Baldwin] The mitochondrial enoyl-CoA hydratase /3-hydroxyacyl-CoA dehydrogenase /3-ketoacyl-CoA thiolase trifunctional protein (trifunctional protein) plays a major role in mitochondrial fatty acid oxidation. The enzyme complex consists of four molecules of α-subunit containing both hydratase and dehydrogenase domains and four molecules of β-subunit containing the thiolase domain. The primary structure of a gastrin-binding protein (GBP) was highly homologous to that of the a-subunit of the trifunctional protein. Here, we report that gastrin inhibits the hydratase, dehydrogenase, and thiolase activities of the trifunctional protein. The gastrin/cholecystokinin receptor antagonist benzotript, which inhibited binding of gastrin to the GBP, also inhibited all three activities of the trifunctional protein. In addition, benzotript inhibits the activities of multifunctional enzymes having similar structures, such as the peroxisomal enoyl-CoA hydratase/3-hydroxyacyl-CoA dehydrogenase bifunctional protein and the Pseudomonas fragi fatty acid oxidation enzyme complex. This reagent, however, hardly inhibited various monofunctional enzymes involved in fatty acid oxidation. © by the Journal of Biochemistry Regular Papers nationallicence benzotript nationallicence fatty acid oxidation nationallicence gastrin nationallicence inhibition nationallicence mitochondrial trifunctional protein nationallicence Hashimoto Takashi Department of Biochemistry, Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto, Nagano 390 aut Shindo Yasuko Department of Biochemistry, Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto, Nagano 390 aut Souri Masayoshi Department of Biochemistry, Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto, Nagano 390 aut Baldwin Graham S. Department of Surgery, Austin Hospital Heidelberg, Victoria 3084, Australia aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1196-1201 0021-924X 119:6<1196 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021368 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021368 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hashimoto Takashi Department of Biochemistry, Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto, Nagano 390 aut NATIONALLICENCE 700 1- Shindo Yasuko Department of Biochemistry, Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto, Nagano 390 aut NATIONALLICENCE 700 1- Souri Masayoshi Department of Biochemistry, Shinshu University School of Medicine 3-1-1 Asahi, Matsumoto, Nagano 390 aut NATIONALLICENCE 700 1- Baldwin Graham S. Department of Surgery, Austin Hospital Heidelberg, Victoria 3084, Australia aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1196-1201 0021-924X 119:6<1196 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford