caa a22 4500 397556268 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021359 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021359 Sequential 1H and 15N NMR Resonance Assignment and Secondary Structure of Ferrocytochrome c2 from Rhodobacter sphaeroides [Elektronische Daten] [Pierre Gans, Jean-Pierre Simorre, Michael Caffrey, Dominique Marion, Pierre Richaud, André Verméglio] Sequence-specific 1H and 15N assignments have been made for the amino acids of the ferrocytochrome c2 from Rhodobacter sphaeroides. Initial assignments were made by analysis of a series of homonuclear 2D COSY, TOCSY, and NOESY spectra obtained with the unlabeled protein. 2D and 3D 1H-15N correlated spectra obtained for a uniformly 15N-labeled ferrocytochrome c2 were used to confirm and extend the assignments. Partial 13C assignments have also been made by means of HSQC experiments on 13C at natural abundance, in particular for about two-thirds of the 13Ca. Medium-range NOE connectivities, together with 3J(HCaNH) coupling constants, indicated the presence of five helices at positions 6-16, 60-68, 74-82, 84-91, and 109-120. No other regular secondary structure was observed. This folding is similar to that previously observed for the ferrocytochrome c2 of Rhodobacter capsulatus in solution, which exhibits approximately 50% sequence identity. Moreover, the rotation rates of the aromatic rings of phenylalanine or tyrosine, when conserved, were similar to those observed for R. capsulatus. Furthermore, Ca chemical shifts, which are sensitive to the secondary structure and ring current effects of the heme, appear to be very similar for the two proteins. Consequently, the solution structure of R. sphaeroides ferrocytochrome c appears to be very similar to that of R. capsulatus ferrocytochrome c2. These results are compared with the X-ray crystal structure of the R. sphaeroides ferrocytochromec2. © by the Journal of Biochemistry Regular Papers nationallicence cytochrome c2 nationallicence electron transfer nationallicence heme protein nationallicence multidimensional NMR nationallicence Rhodobacter sphaeroides nationallicence Gans Pierre Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut Simorre Jean-Pierre Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut Caffrey Michael Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut Marion Dominique Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut Richaud Pierre Laboraioire de Bioénergétique Cellulaire, Département d'Ecophysiologie Végétale et Microbiologie, C.E.N. de Cadarache 13108 Saint-Paul-lez-Durance CEDEX, France aut Verméglio André Laboraioire de Bioénergétique Cellulaire, Département d'Ecophysiologie Végétale et Microbiologie, C.E.N. de Cadarache 13108 Saint-Paul-lez-Durance CEDEX, France aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1131-1142 0021-924X 119:6<1131 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021359 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021359 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Gans Pierre Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut NATIONALLICENCE 700 1- Simorre Jean-Pierre Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut NATIONALLICENCE 700 1- Caffrey Michael Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut NATIONALLICENCE 700 1- Marion Dominique Institut de Biologie Structural CEA-CNRS "Jean-Pierre Ebel” 41 Avenue des Martyrs, 38027 Grenoble CEDEX, France aut NATIONALLICENCE 700 1- Richaud Pierre Laboraioire de Bioénergétique Cellulaire, Département d'Ecophysiologie Végétale et Microbiologie, C.E.N. de Cadarache 13108 Saint-Paul-lez-Durance CEDEX, France aut NATIONALLICENCE 700 1- Verméglio André Laboraioire de Bioénergétique Cellulaire, Département d'Ecophysiologie Végétale et Microbiologie, C.E.N. de Cadarache 13108 Saint-Paul-lez-Durance CEDEX, France aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1131-1142 0021-924X 119:6<1131 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford