caa a22 4500 397556284 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021357 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021357 Identification and Characterization of the acoD Gene Encoding a Dihydrolipoamide Dehydrogenase of the Klebsiella pneumoniae Acetoin Dehydrogenase System [Elektronische Daten] [Hwei-Ling Peng, Wen-Ling Deng, Yin-Hsiu Yang, Hwan-You Chang] The acoD gene, which encodes a dihydrolipoamide dehydrogenase component of the acetoin dehydrogenase enzyme system of Klebsiella pneumoniae was isolated and the nucleotide sequence determined. The gene is capable of encoding a protein of 465 amino acid residues with conserved binding domains for NAD and FAD, and two redox-active cysteine residues. The acoD gene product exhibited a Michaelis constant of 170 μM for NAD, while NADP can not be used as a substrate. The purified enzyme appeared to be a dimer of the acoD gene product. It did not associate tightly with the El and E2 components of either acetoin dehydrogenase or 2-oxoglutarate dehydrogenase to form an active multi-enzyme complex. © by the Journal of Biochemistry Regular Papers nationallicence acetoin dehydrogenase nationallicence aco operon nationallicence dihydrolipoamide dehydrogenase nationallicence Peng Hwei-Ling Departments of Microbiology and Immunology Kwei-San, Taiwan aut Deng Wen-Ling Departments of Microbiology and Immunology Kwei-San, Taiwan aut Yang Yin-Hsiu Molecular and Cellular Biology, Chang-Gung College of Medicine and Technology Kwei-San, Taiwan aut Chang Hwan-You Molecular and Cellular Biology, Chang-Gung College of Medicine and Technology Kwei-San, Taiwan aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1118-1123 0021-924X 119:6<1118 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021357 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021357 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Peng Hwei-Ling Departments of Microbiology and Immunology Kwei-San, Taiwan aut NATIONALLICENCE 700 1- Deng Wen-Ling Departments of Microbiology and Immunology Kwei-San, Taiwan aut NATIONALLICENCE 700 1- Yang Yin-Hsiu Molecular and Cellular Biology, Chang-Gung College of Medicine and Technology Kwei-San, Taiwan aut NATIONALLICENCE 700 1- Chang Hwan-You Molecular and Cellular Biology, Chang-Gung College of Medicine and Technology Kwei-San, Taiwan aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1118-1123 0021-924X 119:6<1118 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford