caa a22 4500 397556314 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199606 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021350 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021350 Pyridine-Promoted Factor- and Energy-Free Peptide Synthesis Systems Prepared from Various Organisms Including Prokaryote, Eukaryote, and Mitochondria [Elektronische Daten] [Takahiko Nojima, Itaru Nitta, Takuya Ueda, Kimitsuna Watanabe] We demonstrate here that ribosomes from not only Escherichia coli and Thermus thermo-philus [Nitta et al. (1994) J. Biochem. 115, 803-807; ibid., (1995) 118, 841-849] but also yeast and bovine mitochondria catalyze peptide synthesis promoted by a high concentration of pyridine in the absence of soluble protein factors and chemical energy sources, and compare some characteristic features of the reactions among these organisms. Sensitivities against antibiotics, chloramphenicol and cycloheximide, showed the same tendency to those in the in vitro aqueous translation systems of these organisms, suggesting that the basic mechanism for peptide synthesis is the same among these organisms. The optimal concentration of pyridine was centered at 50 % for all systems, although the dependencies on the pyridine concentrations and the yields of the products were different from one another. All these systems required Mg2+, and only mitochondrial system showed the extra Mn2+-requirement, which enhanced the yield by several fold. The optimum reaction temperatures coincided closely with the growing temperatures of the organisms except for the mitochondrial system, which showed the highest activity above 80°C. The rationale for these observations remains to be solved. © by the Journal of Biochemistry Regular Papers nationallicence antibiotics nationallicence Mn2+ ion nationallicence peptide synthesis system nationallicence pyridine nationallicence ribosomes nationallicence Nojima Takahiko Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut Nitta Itaru Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut Ueda Takuya Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut Watanabe Kimitsuna Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1076-1079 0021-924X 119:6<1076 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021350 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021350 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nojima Takahiko Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Nitta Itaru Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Ueda Takuya Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Watanabe Kimitsuna Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/6(1996-06), 1076-1079 0021-924X 119:6<1076 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford