caa a22 4500 397556357 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021435 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021435 A Chaperone-Like Function of Intramolecular High-Mannose Chains in the Oxidative Refolding of Bovine Pancreatic RNase B [Elektronische Daten] [Haruki Yamaguchi, Makoto Uchida] This paper describes a chaperone-like function of the intramolecular N-glycans of bovine pancreatic RNase B. We studied air-oxidative regeneration from reductively denatured species of RNase B and its nonglycosylated form, RNase A. RNase B was reactivated much faster than RNase A, while RNase A was liable to aggregate during the regeneration. An Asn-linked oligosaccharide, Man5GlcNA2Asn which corresponds to the most predominant sugar chain (ca. 60%) of RNase B, enhanced the reactivation of the denatured RNases A and B. The stimulatory effect of this Asn-oligosaccharide revealed that the N-glycans of RNase B facilitate the transformation of bulky intermediates into folded, compact species. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence Asn-linked oligosaccharide nationallicence Asn-linked oligosaccharide function nationallicence glycoprotein nationallicence protein folding nationallicence ribonuclease B nationallicence Yamaguchi Haruki Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho Sakai, Osaka 593 aut Uchida Makoto Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho Sakai, Osaka 593 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 474-477 0021-924X 120:3<474 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021435 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021435 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Haruki Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho Sakai, Osaka 593 aut NATIONALLICENCE 700 1- Uchida Makoto Department of Applied Biological Chemistry, College of Agriculture, Osaka Prefecture University, 1-1 Gakuen-cho Sakai, Osaka 593 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 474-477 0021-924X 120:3<474 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford