caa a22 4500 397556365 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021450 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021450 Kinetic and Cross-Linking Studies on the Interactions of Negative Patch Mutant Plastocyanin from Silene pratensis with Photosystem I Complexes from Cyanobacteria, Green Algae, and Plants [Elektronische Daten] [Takashi Hibino, Byung Hyun Lee, Tatsuo Yajima, Akira Odani, Osamu Yamauchi, Teruhiro Takabe] The site-directed mutants of negative patches on silene plastocyanin (PC) were used to investigate the change of interactions between photosystem I (PSI) and PC during the course of evolution from cyanobacteria to plants. The net charges of two highly conserved negative patches (#42-45 and #59-61) on silene PC were systematically modified from −4 to +1. PSI complexes from cucumber and Chlamydomonas reinhardtii were efficient electron acceptors for silene PC. The increase of net charge on the negative patch (#42-45) of silene PC decreased the reduction rates of PSI from cucumber and Chlamydomonas, while the modification of the other negative patch (#59-61) had no effect. Though the addition of MgC12 decreased the reduction rate of cucumber PSI, the decrease was severely diminished in the case of Chlamydomonas PSI, and the reduction rate increased with increasing concentration of MgC12 when the net charge of the negative patch (#42-45) was modified to + 1. The PSI complexes from Anabaena variabilis and Synechosystis sp. PCC 6803 were inefficient electron acceptors for 8ilene PC and their rates were almost independent of the net charge of the negative patches, as well as the ionic strength of the reaction mixtures. Silene PC specifically cross-linked to the PsaF subunit of PSI complexes from cucumber, Chlamydomonas, Anabaena, and Synechosystis sp. PCC 6803. Modification of the negative patch (#42-45) inhibited the formation of cross-linked adducts in all the cases examined, where as modification of the other negative patch (#59-61) had essentially no effect. Based on these results, the changes of electrostatic interactions between PC and PSI during the course of evolution from cyanobacteria to plants are discussed. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence electron transfer nationallicence photosynthesis nationallicence photosystem I nationallicence plastocyanin nationallicence site-directed mutagene8is nationallicence Hibino Takashi Departrnent of Chemistry, Faculty of Science & Technology, Meijo University Tenpaku-hu Nagoya 468 aut Lee Byung Hyun BioScience Center, School of Agricultural Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut Yajima Tatsuo Department of Chemistry, Faculty of Science, Nagoya University Chikusa-k4, Nagoya 464-01 aut Odani Akira Department of Chemistry, Faculty of Science, Nagoya University Chikusa-k4, Nagoya 464-01 aut Yamauchi Osamu Department of Chemistry, Faculty of Science, Nagoya University Chikusa-k4, Nagoya 464-01 aut Takabe Teruhiro Departrnent of Chemistry, Faculty of Science & Technology, Meijo University Tenpaku-hu Nagoya 468 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 556-563 0021-924X 120:3<556 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021450 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021450 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Hibino Takashi Departrnent of Chemistry, Faculty of Science & Technology, Meijo University Tenpaku-hu Nagoya 468 aut NATIONALLICENCE 700 1- Lee Byung Hyun BioScience Center, School of Agricultural Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut NATIONALLICENCE 700 1- Yajima Tatsuo Department of Chemistry, Faculty of Science, Nagoya University Chikusa-k4, Nagoya 464-01 aut NATIONALLICENCE 700 1- Odani Akira Department of Chemistry, Faculty of Science, Nagoya University Chikusa-k4, Nagoya 464-01 aut NATIONALLICENCE 700 1- Yamauchi Osamu Department of Chemistry, Faculty of Science, Nagoya University Chikusa-k4, Nagoya 464-01 aut NATIONALLICENCE 700 1- Takabe Teruhiro Departrnent of Chemistry, Faculty of Science & Technology, Meijo University Tenpaku-hu Nagoya 468 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 556-563 0021-924X 120:3<556 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford