caa a22 4500 397556373 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021452 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021452 Rapid Method of Preparation of Lysoglycosphingolipids and Their Confirmation by Delayed Extraction Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry [Elektronische Daten] [Tamotsu Taketomi, Atsushi Hara, Kei-ichi Uemura, Eiko Sugiyama] We have recently developed a rapid method for the preparation of lysoglycosphingolipids on a small scale, in high yield. This procedure of microwave-mediated saponification of about 1 mg of glycosphingolipid with 0.5 ml of 0.1 M NaOH in methanol for two minutes can be easily repeated if larger amounts of lyso-compounds are needed. We have also found that the new methodology of delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry in the reflector mode is extremely effective for the confirmation of different lysoglycosphingolipids together with their long chain base components. The combined method of preparation and confirmation of lysoglycosphingolipids is also important for the identification of long chain bases of various sphingolipids, because the usual analytical method of long-chain bases of sphingolipids depends on acidic methanolysis, which results in the formation of by-products such as O-methylsphingosines and threo-sphingosines. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence glycosphingoliplds nationallicence lysoglycosphingolipids nationallicence matrix-assisted laser desorption ionization time-of-flight mass spectrometry nationallicence sphingolipids nationallicence sphingosines nationallicence Taketomi Tamotsu Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut Hara Atsushi Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut Uemura Kei-ichi Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut Sugiyama Eiko Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 573-579 0021-924X 120:3<573 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021452 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021452 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Taketomi Tamotsu Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut NATIONALLICENCE 700 1- Hara Atsushi Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut NATIONALLICENCE 700 1- Uemura Kei-ichi Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut NATIONALLICENCE 700 1- Sugiyama Eiko Department of Biochemistry, Division of Neuro-Aging, Research Center on Aging and Adaptation, Shinshu University School of Medicine, Matsumoto, Nagano 390 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 573-579 0021-924X 120:3<573 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford