caa a22 4500 397556381 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021439 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021439 Phorbol Ester, but Not Endotoxin, Desensitizes Mannan-Induced Glycogenolysis in the Perfused Rat Liver [Elektronische Daten] [Kazuhiro Kimura, Miyuki Hamada, Mitsuaki Moriyama, Yukiko Kannan, Masakazu Shiota, Keisuke Sakurada, Manabu Musashi, Tsukasa Sugano] Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupifer cells by endotoxin and phorbol ester alters the glycogenolytic responses to mannan. Infusion of lipopolysaccharide (LPS, 10 ,μg/ ml) in the perfusate failed to inhibit the responses to mannan. Intravenous administration of LPS (1 mg/kg) 6 and 24 h before perfusion did not desensitize the responses tomannan, suggesting that the responses through mannose/GlcNAc receptors in the liver are retained even after activation of Kupffer cells by LPS. In contrast, prior infusion of phorbol 12-myristate 13-acetate (PMA, 100 nM) in vitro abolished the glycogenolytic responses to subsequently infused mannan, but not that to norepinephrine (100 nM), while prior infusions of 4-α-phorbol 12,13-didecanoate (100 nM), A23187 (50 nM), or forskolin (1 μM) had no effect on the mannan-induced responses. H-7, an inhibitor of protein kinase C, reduced the glycogenolytic responses to mannan, while it failed to restore the desensitization. These results suggest that protein kinase C may be involved in the process of glycogenolysis by mannan, but is unlikely to be involved in the homologous desensitization of the responses. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence LPS nationallicence mannose/GlcNAc receptor nationallicence protein kinase C nationallicence Kimura Kazuhiro Department of Biomedical Sciences, Hokkaido University School of Veterinary Medicine Kite-ku, Sapporo 060 aut Hamada Miyuki Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut Moriyama Mitsuaki Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut Kannan Yukiko Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut Shiota Masakazu Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut Sakurada Keisuke Third Department of Internal Medicine, Hokkaido University School of Medicine Kita-ku, Sapporo 060 aut Musashi Manabu Third Department of Internal Medicine, Hokkaido University School of Medicine Kita-ku, Sapporo 060 aut Sugano Tsukasa Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 488-493 0021-924X 120:3<488 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021439 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021439 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kimura Kazuhiro Department of Biomedical Sciences, Hokkaido University School of Veterinary Medicine Kite-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Hamada Miyuki Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut NATIONALLICENCE 700 1- Moriyama Mitsuaki Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut NATIONALLICENCE 700 1- Kannan Yukiko Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut NATIONALLICENCE 700 1- Shiota Masakazu Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut NATIONALLICENCE 700 1- Sakurada Keisuke Third Department of Internal Medicine, Hokkaido University School of Medicine Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Musashi Manabu Third Department of Internal Medicine, Hokkaido University School of Medicine Kita-ku, Sapporo 060 aut NATIONALLICENCE 700 1- Sugano Tsukasa Department of Veterinary Physiology, College of Agriculture, Osaka Prefecture University Sakai Osaka 591 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 488-493 0021-924X 120:3<488 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford