caa a22 4500 397556403 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021437 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021437 Crystallization and Preliminary X-Ray Diffraction Studies of N-Acyl-D-glucosamine 2-Epimerase from Porcine Kidney [Elektronische Daten] [Isafumi Maru, Jun Ohnish, Yasuhiro Ohta, Wataru Hashimoto, Yoji Tsukada, Murata Kousaku, Bunzo Mikami] N-Acyl-D-glucosamine 2-epimerase from porcine kidney, which was cloned and expressed in Escherichia coli, was crystallized by the vapor-diffusion method, using polyethylene glycol and ammonium acetate as precipitants. The crystals were resistant to X-ray radiation damage and diffracted to more than 2.0 Å resolution. The diffraction pattern indicated that the crystals belong to the orthorhombic system, space group P21,21 21 with unit-cell dimensions of a=78.1, b=97.2, and c=100.7 Å. It is supposed that the asymmetric unit consists of two N-acyl-D-glucosamine 2-epimerase molecules. Collection of data on the native crystals indicated that they are suitable for X-ray structural analysis. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence N-acyl-D-glucosamine 2-epimerase nationallicence crystallization nationallicence porcine kidney nationallicence reninbinding protein nationallicence X-ray crystallography nationallicence Maru Isafumi Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut Ohnish Jun Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut Ohta Yasuhiro Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut Hashimoto Wataru Research Institute for Food Science Kyoto University Uji, Kyoto 611 aut Tsukada Yoji Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut Kousaku Murata Research Institute for Food Science Kyoto University Uji, Kyoto 611 aut Mikami Bunzo Research Institute for Food Science Kyoto University Uji, Kyoto 611 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 481-482 0021-924X 120:3<481 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021437 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021437 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Maru Isafumi Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Ohnish Jun Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Ohta Yasuhiro Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Hashimoto Wataru Research Institute for Food Science Kyoto University Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Tsukada Yoji Kyoto Research Laboratories, Marukin Shoyu Co., Ltd. Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Kousaku Murata Research Institute for Food Science Kyoto University Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Mikami Bunzo Research Institute for Food Science Kyoto University Uji, Kyoto 611 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 481-482 0021-924X 120:3<481 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford