caa a22 4500 397556446 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021449 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021449 Novel Actin Depolymerizing Macrolide Aplyronine A [Elektronische Daten] [Shin-ya Saito, Shugo Watabe, Hiroshi Ozaki, Hideo Kigoshi, Kiyoyuki Yamada, Nobuhiro Fusetani, Hideaki Karaki] Aplyronine A is a macrolide isolated from Aplysia kurodai. By monitoring fluorescent intensity of pyrenyl-actin, it was found that aplyronine A inhibited both the velocity and the degree of actin polymerization. Aplyronine A also quickly depolymerized F-actln. The kinetics of depolymerizatlon suggest that aplyronine A severs F-actin. The relationship between the concentration of total actin and F-actin at different concentrations of aplyronine A suggests that aplyronine A forms a 1:1 complex with G-actin. From these results, it is concluded that aplyronine A inhibits actin polymerization and depolymerizes F-actin by nibbling. Comparison of the chemical structure of aplyronine A and another actin-depolymerizing macrolide, mycalolide B, suggests that the side-chain but not the macrolide ring of aplyronlne A may account for its actin binding and severing activity. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence actin nationallicence actin depolymerization nationallicence aplyronine A nationallicence macrolide nationallicence mycalolide B nationallicence lead nitrate nationallicence Saito Shin-ya Department of Veterinary Pharmacology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut Watabe Shugo Laboratory of Aquatic Biotechnology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut Ozaki Hiroshi Department of Veterinary Pharmacology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut Kigoshi Hideo Departrnent of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464 aut Yamada Kiyoyuki Departrnent of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464 aut Fusetani Nobuhiro Laboratory of Marine Biochemistry Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut Karaki Hideaki Department of Veterinary Pharmacology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 552-555 0021-924X 120:3<552 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021449 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021449 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Saito Shin-ya Department of Veterinary Pharmacology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Watabe Shugo Laboratory of Aquatic Biotechnology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Ozaki Hiroshi Department of Veterinary Pharmacology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Kigoshi Hideo Departrnent of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464 aut NATIONALLICENCE 700 1- Yamada Kiyoyuki Departrnent of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464 aut NATIONALLICENCE 700 1- Fusetani Nobuhiro Laboratory of Marine Biochemistry Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Karaki Hideaki Department of Veterinary Pharmacology Graduate School of Agricultere and Life Sciences, The University of Tokyo, 1-1-1 Yayoi Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 552-555 0021-924X 120:3<552 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford