caa a22 4500 397556462 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021465 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021465 Spliceosomal Introns in Conserved Sequences of U1 and U5 Small Nuclear RNA Genes in Yeast Rhodotorula hasegawae [Elektronische Daten] [Yuzi Takahashi, Tokio Tani, Yasumi Ohsima] U1, U2, U4, U5, and U6 small nuclear RNAs are essential for precursor mRNA splicing. We previously found one and four mRNA-type, or spliceosomal introns, in the U2 and U6 genes, respectively, of yeast Rhodotorula hasegawae (Erythrobasidium hasegawianum). We proposed that U2 and U6 RNAs form a catalytic core for precursor mRNA splicing and that the introns in those small nuclear RNA genes may have been acquired through reverse splicing of an intron from a precursor mRNA into a catalytic site in small nuclear RNAs. In the present study, we analyzed U1, U4, and U5 genes in R. hasegawae. One spliceosomal intron was found in the U1 region forming base-pairs with a 5′ splice site of a precursor mRNA. The U5 gene has two spliceosomal introns in the region that interacts with 5′ and 3′ splice sites. In contrast, the gene for U4 RNA, which is released from the spliceosome prior to the first step of the splicing reaction, has no intron. These results lend a further support to the proposed relation between presence and position of an intron in an small nuclear RNA gene and the function of the encoded small nuclear RNA. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence pre-mRNA splicing nationallicence spliceosomal intron nationallicence U1 snRNA nationallicence U4 snRNA nationallicence U5 snRNA nationallicence Takahashi Yuzi Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-81 aut Tani Tokio Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-81 aut Ohsima Yasumi Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-81 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 677-683 0021-924X 120:3<677 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021465 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021465 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Yuzi Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-81 aut NATIONALLICENCE 700 1- Tani Tokio Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-81 aut NATIONALLICENCE 700 1- Ohsima Yasumi Department of Biology, Faculty of Science, Kyushu University, Hakozaki, Higashi-ku, Fukuoka 812-81 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 677-683 0021-924X 120:3<677 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford