caa a22 4500 397556500 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021438 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021438 Molecular Cloning and Gene Expression of Chum Salmon Cystatin [Elektronische Daten] [Michiaki Yamashita, Shiro Konagaya] A salmon cystatin cDNA clone was isolated from a chum salmon cDNA library. The clone encoded a full-length extracellular-type cystatin and its signal peptide and included 5′- and 3′ -untranslated regions. The deduced amino acid sequence showed a high degree of sequence similarity to mammalian cystatin C, chicken egg cystatin, and chum salmon pituitary cystatin. By Northern blot analysis, the salmon cystatin was found to show apparently non-tissue specific expression. Because platyfish EHS cells transfected with a cystatin expression vector produced a 13 kDa mature cystatin in the culture medium, the salmon cystatin was considered to act as an extracellular type of cystatin in the fish cells. These findings indicate that the salmon cystatin is a homolog of mammalian cystatin C. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence chum salmon nationallicence cystatin nationallicence cysteine protease nationallicence Yamashita Michiaki National Research Institue of Fisheries Science, 2-12-4 Fukuuro, Kanazawa-ku, Yokohama 236 aut Konagaya Shiro National Research Institue of Fisheries Science, 2-12-4 Fukuuro, Kanazawa-ku, Yokohama 236 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 483-487 0021-924X 120:3<483 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021438 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021438 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamashita Michiaki National Research Institue of Fisheries Science, 2-12-4 Fukuuro, Kanazawa-ku, Yokohama 236 aut NATIONALLICENCE 700 1- Konagaya Shiro National Research Institue of Fisheries Science, 2-12-4 Fukuuro, Kanazawa-ku, Yokohama 236 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 483-487 0021-924X 120:3<483 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford