caa a22 4500 397556527 CHVBK 20180308164803.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021442 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021442 The Crystal Structure of Lipase II from Rhizopus niveus at 2.2 Å Resolution [Elektronische Daten] [Mitsutaka Kohno, Jiro Funatsu, Bunzo Mikami, Wataru Kugimiya, Takaharu Matsuo, Yuhei Marita] The crystal and molecular structure of Lipase II from Rhizopus niveus was analyzed using X-ray single crystal diffraction data at a resolution of 2.2 Å. The structure was refined than R-factor of 0.19 for all available data. This lipase was purified and crystallized as Lipase I, which contains two polypeptide chains combined through non-covalent interaction. However, during crystal growth, Lipase I was converted to Lipase II, which consists of a single polypeptide chain of 269 amino acid residues, by limited proteolysis. The structure of Lipase II shows a typical α/β hydrolase fold containing the so-called nucleophilic elbow. The catalytic center of this enzyme is analogous to those of other neutral lipases and serine proteases. This catalytic center is sheltered by an α-helix lid, which appears in neutral lipases, opening the active site at the oil-water interface. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence catalytic triad nationallicence crystal structure nationallicence hydrolase fold nationallicence Rhizopus niveus nationallicence triacylglycerol acylhydrolase nationallicence Kohno Mitsutaka Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut Funatsu Jiro Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut Mikami Bunzo Research Institute for Food Science, Kyoto University Kyoto University, Uji, Kyoto 611 aut Kugimiya Wataru Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut Matsuo Takaharu Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut Marita Yuhei Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 505-510 0021-924X 120:3<505 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021442 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021442 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kohno Mitsutaka Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Funatsu Jiro Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Mikami Bunzo Research Institute for Food Science, Kyoto University Kyoto University, Uji, Kyoto 611 aut NATIONALLICENCE 700 1- Kugimiya Wataru Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Matsuo Takaharu Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 700 1- Marita Yuhei Central Research Institute Tsukuba R&D Center, Fuji Oil Co., Ltd. 4-3 Kinunodai Yawara, Tsukuba-gun, Ibaraki 300-24 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 505-510 0021-924X 120:3<505 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford