caa a22 4500 397556551 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021448 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021448 Lead Nitrate Inhibits the Induction of CYP1A mRNAs by Aromatic Amines but not by Aryl Hydrocarbons in the Rat Liver [Elektronische Daten] [Masakuni Degawa, Ken-ichi Matsuda, Hiroshi Arai, Yoshiyuki Hashimoto] The effects of lead nitrate on the induction of hepatic cytochrome P4501A (CYP1A) isoforms, mainly CYP1A2, by aromatic amines (2-methoxy-4-aminoazobenzene, 2-amino-3-methyl-9H-pyrido [2,3-b indole and 2-amino-1-methyl-6-phenylimidazo [4,5-b] pyridine) and aryl hydrocarbons (3-methyicholanthrene, benzo[α] and β-naphthoflavone) in male F344 rats were examined at the levels of mRNA, protein and activity of the enzymes. Pretreatment of rats with lead nitrate suppressed the expression of hepatic CYP1A enzyme(s), especially CYP1A2, at both levels of protein and activity of the enzyme(s) by treatment with an aromatic amine or an aryl hydrocarbon. On the other hand, the lead nitrate pretreatment suppressed the induction of CYP1A mRNA(s) by an aromatic amine but not by an aryl hydrocarbon. These findings indicate that lead nitrate suppresses the expression of CYP1A enzymes at both stages of post-translation of mRNAs and transcriptional activation of the genes, and further suggest that the pathway for the transcriptional activation of the CYP1A genes by the aromatic amines is different from that by the aryl hydrocarbons. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence aromatic amine nationallicence aryl hydrocarbon nationallicence hydrogen peroxide nationallicence CYP1A nationallicence cytochrome P450 nationallicence lead nitrate nationallicence Degawa Masakuni Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut Matsuda Ken-ichi Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut Arai Hiroshi Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut Hashimoto Yoshiyuki Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 547-551 0021-924X 120:3<547 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021448 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021448 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Degawa Masakuni Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut NATIONALLICENCE 700 1- Matsuda Ken-ichi Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut NATIONALLICENCE 700 1- Arai Hiroshi Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut NATIONALLICENCE 700 1- Hashimoto Yoshiyuki Faculty of Pharmaceutical Sciences, Tohoku University, Aramaki-aza-Aoba, Aoba-ku Sendai 980-77 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 547-551 0021-924X 120:3<547 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford