caa a22 4500 39755656X CHVBK 20180308164804.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021461 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021461 The Primary Structure of the Major Pepsinogen from the Gastric Mucosa of Tuna Stomach [Elektronische Daten] [Masao Tanji, Etsuko Yakabe, Takashi Kageyama, Kenji Takahashi] The complete primary structure of the major component of tuna pepsinogens was determined by conventional protein chemistry methods. It was composed of a prosegment of 37 residues and a pepsin moiety of 323 residues, having a relative molecular mass of 39,364. The essential aspartyl residues in the active site and the three disulnde bonds common to other pepsinogens were conserved; however, several unique substitutions and/or deletions characteristic of tuna pepsinogen were found at various positions, especially in the prosegment and subsite regions, as compared with the sequences of other pepsinogens, which may affect the rate of activation of the zymogen, and/or the catalytic function and substrate specificity of the enzyme. Tuna pepsinogen is the least acidic among pepsinogens. The sequence identity between tuna pepsinogen and other pepsinogens ranged from 45 to 52%. A phylogenetic tree based on the primary structures suggested that tuna pepsinogen diverged from the pepsinogen A and prochymosin groups in an early period of pepsinogen evolution. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence amino acid sequence nationallicence pepsin nationallicence pepsinogen nationallicence phylogenetic tree nationallicence tuna nationallicence Tanji Masao Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo Bunkyo-ku, Tokyo 113 aut Yakabe Etsuko Department of Chemistry, Faculty of Science and Technology, Aoyama Gakuin University Setagaya-ku, Tokyo 157 aut Kageyama Takashi Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University Inuyama, Aichi 484 aut Takahashi Kenji Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 647-656 0021-924X 120:3<647 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021461 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021461 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tanji Masao Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Yakabe Etsuko Department of Chemistry, Faculty of Science and Technology, Aoyama Gakuin University Setagaya-ku, Tokyo 157 aut NATIONALLICENCE 700 1- Kageyama Takashi Department of Cellular and Molecular Biology, Primate Research Institute, Kyoto University Inuyama, Aichi 484 aut NATIONALLICENCE 700 1- Takahashi Kenji Department of Biophysics and Biochemistry, Graduate School of Science, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 647-656 0021-924X 120:3<647 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford