caa a22 4500 397556578 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021440 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021440 Cloning and Expression of cDNA Encoding a Human Ubiquitin-Conjugating Enzyme Similar to the Drosophila bendless Gene Product [Elektronische Daten] [Tomoko Yamaguchi, Nam-Soon Kim, Shingo Sekine, Hiroaki Seino, Fumio Osaka, Fumiaki Yamao, Seishi Kato] cDNA encoding a novel human ubiquitin-conjugating enzyme has been cloned from an epidermoid carcinoma KB cDNA library. This clone encodes a protein of 152 amino acids with a calculated Mr of 17,137. The amino acid sequence showed 80% identity with the Drosophila's bendless gene product (ubiquitin-conjugating enzyme E2). The corresponding transcripts are highly expressed in heart, skeletal muscle, and testis. The product expressed in Escherichia coli exhibited the ability to form a thiol ester linkage with ubiquitin in a ubiquitin-activating enzyme El-dependent manner. These results suggest that the obtained cDNA encodes a novel human E2 which may be involved in protein degradation mainly in the muscles and testis. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence bacterial expression nationallicence cDNA nationallicence human homolog nationallicence ubiquitin-conjugating enzyme nationallicence Yamaguchi Tomoko Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut Kim Nam-Soon Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut Sekine Shingo Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut Seino Hiroaki National Institute of Genetics Mishima, Shizuoka 411 aut Osaka Fumio National Institute of Genetics Mishima, Shizuoka 411 aut Yamao Fumiaki National Institute of Genetics Mishima, Shizuoka 411 aut Kato Seishi Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 494-496 0021-924X 120:3<494 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021440 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021440 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaguchi Tomoko Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut NATIONALLICENCE 700 1- Kim Nam-Soon Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut NATIONALLICENCE 700 1- Sekine Shingo Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut NATIONALLICENCE 700 1- Seino Hiroaki National Institute of Genetics Mishima, Shizuoka 411 aut NATIONALLICENCE 700 1- Osaka Fumio National Institute of Genetics Mishima, Shizuoka 411 aut NATIONALLICENCE 700 1- Yamao Fumiaki National Institute of Genetics Mishima, Shizuoka 411 aut NATIONALLICENCE 700 1- Kato Seishi Sagarni Chemical Research Center 4-4-1 Nishi-Ohnuma, Sagamihara Kanagawa 229 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 494-496 0021-924X 120:3<494 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford