caa a22 4500 397556586 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199609 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021447 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021447 Free Radical-Independent Protection by Nerve Growth Factor and Bcl-2 of PC12 Cells from Hydrogen Peroxide-Triggered Apoptosis [Elektronische Daten] [Takumi Satoh, Naoto Sakai, Yasushi Enokido, Yasuo Uchiyama, Hiroshi Hatanaka] To investigate the mechanism of oxidative stress-induced death of PC12 cells, we performed confocal and flow cytometric analysis with a reactive oxygen species (ROS)-specific fluorogen, 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate, di(acetoxymethyl ester) (C-DCDHIF-DA). Hydrogen peroxide significantly decreased the number of viable PC12 cells after 24 h. Hydrogen peroxide caused membrane blebbing, nuclear condensation and DNA fragmentation, indicating that the PC12 cells died due to apoptosis. The hydrogen peroxidetriggered apoptosis of PC12 cells was associated with enhanced ROS production in a dose-dependent manner by measuring with C-DCDHF-DA. Nerve growth factor (NGF) and Bcl-2 inhibited the hydrogen peroxide-induced apoptosis of PC12 cells. Neither of them, however, reduced the ROS production in PC12 cells. These data suggest that NGF or Bcl-2 protects PC12 cells from hydrogen peroxide-triggered apoptosis independently from ROS production. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence apoptosis nationallicence Bcl-2 nationallicence hydrogen peroxide nationallicence NGF nationallicence reactive oxygen species nationallicence Satoh Takumi Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut Sakai Naoto Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut Enokido Yasushi Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut Uchiyama Yasuo Department of Anatomy, Medical School of Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut Hatanaka Hiroshi Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 120/3(1996-09), 540-546 0021-924X 120:3<540 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021447 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021447 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Satoh Takumi Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Sakai Naoto Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Enokido Yasushi Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Uchiyama Yasuo Department of Anatomy, Medical School of Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Hatanaka Hiroshi Division of Protein Biosynthesis, Institute for Protein Research, Osaka University 3-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/3(1996-09), 540-546 0021-924X 120:3<540 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford