caa a22 4500 39755673X CHVBK 20180308164804.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021411 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021411 In Vivo Survival and Organ Uptake of Loaded Carrier Rat Erythrocytes [Elektronische Daten] [F. Javier Alvarez, Angel Herráiez, Juan C. Murciano, José A. Jordán, José C. Díez, M. Cristina Tejedor] Rat RBCs loaded with125 I-CA by hypotonic dialysis and isotonic resealing were evaluated as a carrier system. Loaded RBCs stored at 4°C remained unlysed (90% survival) allowing release of encapsulated126 I-CA for up to 4 days. Thereafter, cellular lysis increased significantly. IP-injected loaded RBCs reached the maximum level (50%) in circulation at 24 h post-injection. Circulating loaded RBCs showed a half-life of 8-10 days, which was advantageous for carrier function. In contrast to IP-injected free CA, which remained in circulation for only a short time, encapsulated CA showed significant levels in circulation up to 10 days post-injection. The profile of organ uptake with time is essentially not altered for loaded with respect to native cells, being higher the removal of loaded cells and mainly localized in spleen. Nevertheless, liver is the organ with highest elimination capacity for both native and loaded cells, showing its maximum at 24 h post-injection. Concomitantly, the concentration of125 I-CA in all organs studied was highest at this time. These data demonstrate that rat loaded RBCs can potentially be used as a carrier system for long-term dissemination of drug into the organism, with specially increased delivery to the spleen. They also support the use of the rat as an experimental model for biochemical and pharmacological studies in these therapeutic systems. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence carrier erythrocytes nationallicence cell targeting nationallicence encapsulated carbonic anhydrase nationallicence organ uptake nationallicence sustained release nationallicence Alvarez F. Javier Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut Herráiez Angel Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut Murciano Juan C. Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut Jordán José A. Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut Díez José C. Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut Tejedor M. Cristina Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 286-291 0021-924X 120:2<286 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021411 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021411 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Alvarez F. Javier Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut NATIONALLICENCE 700 1- Herráiez Angel Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut NATIONALLICENCE 700 1- Murciano Juan C. Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut NATIONALLICENCE 700 1- Jordán José A. Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut NATIONALLICENCE 700 1- Díez José C. Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut NATIONALLICENCE 700 1- Tejedor M. Cristina Departamento de Bioquĺmica y Biolog´ Molecular, Facultad de Medicina, Universidad de Alcald de Henares, 28871-Alcala de Henares, Madrid, Spam aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 286-291 0021-924X 120:2<286 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford