caa a22 4500 397556780 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021416 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021416 Single-Strand Conformation Polymorphism (SSCP) Can Be Explained by Semistable Conformation Dynamics of Single-Stranded DNA [Elektronische Daten] [Yoichi Nakabayashi, Koichi Nishigaki] The cause of separation in gel electrophoresis between highly homologous ss (singlestranded) DNAs as observed in SSCP (single-strand conformation polymorphism) was pursued. Advancing our previous explanation [J. Biochem. 99, 663-671 (1986)], the mobility difference of ssDNAs was correlated to differences in their dynamic conformation (not to differences in their most stable structure), focusing on point-substituted sites. The contribution of semistable conformation dynamics was considered to be critical. Putative factors which may influence mobility (i.e., length of ssDNAs, location of substituted sites, and types of substitutions) were experimentally examined and critically discussed. Understanding of these phenomena should yield improvements in various techniques, such as SSCP, and in evaluation of the solution structures of DNA. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence conformation dynamics nationallicence point mutation nationallicence SSCP nationallicence ssDNA nationallicence solution structure nationallicence Nakabayashi Yoichi Department of Environmental Chemistry and Department of Functional Materials Science, Saitama University, 255 Shimo-Okubo Urawa, Saitama 338 aut Nishigaki Koichi Department of Environmental Chemistry and Department of Functional Materials Science, Saitama University, 255 Shimo-Okubo Urawa, Saitama 338 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 320-325 0021-924X 120:2<320 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021416 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021416 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nakabayashi Yoichi Department of Environmental Chemistry and Department of Functional Materials Science, Saitama University, 255 Shimo-Okubo Urawa, Saitama 338 aut NATIONALLICENCE 700 1- Nishigaki Koichi Department of Environmental Chemistry and Department of Functional Materials Science, Saitama University, 255 Shimo-Okubo Urawa, Saitama 338 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 320-325 0021-924X 120:2<320 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford