caa a22 4500 397556799 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021413 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021413 Open Reading Frame 3 of the Barotolerant Bacterium Strain DSS12 Is Complementary with cydD in Escherichia coli: cydD Functions Are Required for Cell Stability at High Pressure [Elektronische Daten] [Chiaki Kato, Hideyuki Tamegai, Akihiko Ikegami, Ron Usami, Koki Horikoshi] Escherichia coli strain JD518, a cydD-deficient mutant, displayed temperature-sensitive and pressure-sensitive growth. The defective cydD gene in this strain was complemented by open reading frame 3 (ORF3), previously identified in DNA from a barotolerant bacterium, strain DSS12, allowing growth of the cydD mutant under high temperature and high pressure conditions. Spectrophotometrical analysis indicated that the cytochrome bd complex which is assembled by the CydD protein was expressed in E. coli strain JD518 carrying the ORF3 gene at the same level as occurred in the wild-type strain. Our results indicate that the cydD gene functions are required for cell stability under the condition of high pressure stress in bacteria. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence barotolerant bacterium nationallicence cydD nationallicence Escherichia coli nationallicence high pressure nationallicence respiratory system nationallicence Kato Chiaki The DEEP STAR group, Japan Marine Science and Technology Center 2-15 Natsushima-cho, Yokosuka, Kanagawa 237 aut Tamegai Hideyuki The DEEP STAR group, Japan Marine Science and Technology Center 2-15 Natsushima-cho, Yokosuka, Kanagawa 237 aut Ikegami Akihiko Department of Applied Chemistry, Faculty of Engineering, Toyo University 2100 Kujirai, Kawagoe, Saitama 350 aut Usami Ron Department of Applied Chemistry, Faculty of Engineering, Toyo University 2100 Kujirai, Kawagoe, Saitama 350 aut Horikoshi Koki The DEEP STAR group, Japan Marine Science and Technology Center 2-15 Natsushima-cho, Yokosuka, Kanagawa 237 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 301-305 0021-924X 120:2<301 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021413 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021413 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kato Chiaki The DEEP STAR group, Japan Marine Science and Technology Center 2-15 Natsushima-cho, Yokosuka, Kanagawa 237 aut NATIONALLICENCE 700 1- Tamegai Hideyuki The DEEP STAR group, Japan Marine Science and Technology Center 2-15 Natsushima-cho, Yokosuka, Kanagawa 237 aut NATIONALLICENCE 700 1- Ikegami Akihiko Department of Applied Chemistry, Faculty of Engineering, Toyo University 2100 Kujirai, Kawagoe, Saitama 350 aut NATIONALLICENCE 700 1- Usami Ron Department of Applied Chemistry, Faculty of Engineering, Toyo University 2100 Kujirai, Kawagoe, Saitama 350 aut NATIONALLICENCE 700 1- Horikoshi Koki The DEEP STAR group, Japan Marine Science and Technology Center 2-15 Natsushima-cho, Yokosuka, Kanagawa 237 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 301-305 0021-924X 120:2<301 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford