caa a22 4500 397556802 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021432 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021432 Characterization of Yeast sar1 Temperature-Sensitive Mutants, Which Are Defective in Protein Transport from the Endoplasmic Reticulum [Elektronische Daten] [Tomoko Yamanush, Aiko Hirata, Toshihiko Oka, Akihiko Nakano] SAR1 encodes a low molecular weight GTPase that is essential in the early pracess of vesicular transport in the secretory pathway. By random and site-directed mutagenesis of the SAR1 gene, we have obtained three temperature-sensitive mutants, N1321, E112K, and D32G. They all show a defect in transport from the endoplasmic reticulum to the Golgi apparatus, and accumulate endoplasmic reticulum membranes at the restrictive temperature. This is consistent with our previous observations in viuo on a galactose-shutoff mutant as well as the in vitro results, and provides powerful tools for further genetic analyses. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Golgi apparatus nationallicence protein secretion nationallicence small GTPase nationallicence Saccharomyces cereuisiae nationallicence vesicular transport nationallicence Yamanush Tomoko Department of Biological Science, Graduate School of Science The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut Hirata Aiko Institute of Molecular and Cellular Bioscience, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut Oka Toshihiko Department of Biological Science, Graduate School of Science The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut Nakano Akihiko Department of Biological Science, Graduate School of Science The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 452-458 0021-924X 120:2<452 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021432 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021432 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamanush Tomoko Department of Biological Science, Graduate School of Science The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Hirata Aiko Institute of Molecular and Cellular Bioscience, The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Oka Toshihiko Department of Biological Science, Graduate School of Science The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Nakano Akihiko Department of Biological Science, Graduate School of Science The University of Tokyo Hongo, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 452-458 0021-924X 120:2<452 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford