caa a22 4500 397556837 CHVBK 20180308164804.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021414 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021414 Effects of Transfer of Hybridomas Producing Various Isotypes of Immunoglobulins on the C1q Metabolism in Mice [Elektronische Daten] [Takako Sasaki, Hideki Yamada, Hideto Matsui, Yoshiko Dohi, Youki Hirakawa, Teruko Sakanashi, Kunio Yonemasu] In order to elucidate possible effects of immunoglobulin on Clq metabolism at the anabolic steps, serum Clq levels and Clq mRNA of peritoneal exudate cells (PEC) and spleen cells were measured in female BALB/c mice implanted intraperitoneally with complements-fixing IgG2b- or non-C-fixing class IgG3-producing hybridomas and/or with immunoglobulin-non-productive myeloma cells (p3×63-Ag.8.653) (myeloma 653) (2 × 106/0.2 ml) or without any treatment as controls. In the IgG2b-hybridoma-treated mice, the serum C1q levels and C1q mRNA in PEC increased conspicuously as compared with those in the controls, but Clq mRNA in spleen cells was almost equal to that in the control mice. On the other hand, in the IgG3-hybridoma-treated mice, the serum Clq levels decreased significantly, but the extent of such decrease and the level of Clq mRNA in their PEC were almost equivalent to those in the myeloma 653-implanted mice. The serum Clq levels and Clq mRNA in PEC fluctuated similarly in mice injected intraperitoneally with highly purified IgG2b and/or IgG3 preparations. These results suggest some anabolic interaction, as well as catabolic interaction, between the C-fixing class of immunoglobulin and C1q. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence IgG2b- or IgG3-producing hybridoma nationallicence isotype dependent serum Clq-fluctuation nationallicence mouse C1q metabolism nationallicence mRNA of the C1q B-chain nationallicence Sasaki Takako Department of Bacteriology 840 Shijyocho, Kashihara, Nara 634 aut Yamada Hideki Department of Bacteriology 840 Shijyocho, Kashihara, Nara 634 aut Matsui Hideto Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut Dohi Yoshiko Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut Hirakawa Youki Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut Sakanashi Teruko Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut Yonemasu Kunio Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 306-312 0021-924X 120:2<306 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021414 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021414 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Takako Department of Bacteriology 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 700 1- Yamada Hideki Department of Bacteriology 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 700 1- Matsui Hideto Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 700 1- Dohi Yoshiko Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 700 1- Hirakawa Youki Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 700 1- Sakanashi Teruko Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 700 1- Yonemasu Kunio Department of Public Health, Nara Medical University 840 Shijyocho, Kashihara, Nara 634 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 306-312 0021-924X 120:2<306 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford