caa a22 4500 397556853 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021404 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021404 Human UDP-Galactose Translocator: Molecular Cloning of a Complementary DNA That Complements the Genetic Defect of a Mutant Cell Line Deficient in UDP-Galactose Translocator [Elektronische Daten] [Nobuhiko Miura, Nobuhiro Ishida, Masato Hoshino, Masatake Yamauchi, Takahiko Hara, Dai Ayusawa, Masao Kawakita] We have cloned a cDNA that codes for a putative human UDP-galactose translocator (UGT) protein. The cDNA contained an open reading frame of 1,179 base pairs encoding a novel protein of 393 amino acids. Introduction of the open reading frame sequence into a UGT-deficient mouse cell line, Had-1, complemented the genetic defect of the mutant, namely the inability to transport UDP-galactose from the cytosol to the Golgi lumen, as judged from the lectin-sensitivity spectrum of the transformant. To our knowledge, this is the first mammalian nucleotide-sugar translocator whose cDNA sequence has been described. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence CDC91 nationallicence Golgi apparatus nationallicence UDP-galactose nationallicence UDP-galactose translocator nationallicence ZK370.7 nationallicence Miura Nobuhiko Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut Ishida Nobuhiro Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut Hoshino Masato Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut Yamauchi Masatake Division of Genetics, National Institute of Radiological Sciences 4-9-1 Anagawa, Inage-ku, Chiba 260 aut Hara Takahiko Institute of Molecular and Cellular Biosciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut Ayusawa Dai Kihara Institute of Biological Research, Yokohama City University, Maioka-cho Totsuka-ku, Yokohama 244 aut Kawakita Masao Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 236-241 0021-924X 120:2<236 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021404 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021404 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Miura Nobuhiko Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Ishida Nobuhiro Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Hoshino Masato Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Yamauchi Masatake Division of Genetics, National Institute of Radiological Sciences 4-9-1 Anagawa, Inage-ku, Chiba 260 aut NATIONALLICENCE 700 1- Hara Takahiko Institute of Molecular and Cellular Biosciences, The University of Tokyo Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Ayusawa Dai Kihara Institute of Biological Research, Yokohama City University, Maioka-cho Totsuka-ku, Yokohama 244 aut NATIONALLICENCE 700 1- Kawakita Masao Department of Physiological Chemistry, The Tokyo Metropolitan Institute of Medical Science (Rinshoken) 18-22 Honkomagome 3-chome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 236-241 0021-924X 120:2<236 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford