caa a22 4500 397556861 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021431 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021431 Novel Peptide Ligands for Integrin α6β1 Selected from a Phage Display Library [Elektronische Daten] [Ohoshi Murayama, Hitoshi Nishida, Kiyotoshi Sekiguchi] Integrin α6β1 is a major adhesion receptor for the basement membrane, specifically binding to laminin-1. To identify the peptide sequences recognized by α6β1, we screened a 15-mer phage display library by panning with α6β1 purified from human placenta. DNA sequencing of 73 randomly picked phage revealed that three clones were dominantly enriched after repeated panning with α6β1. None of the peptide sequences displayed on these phage showed significant homology to laminin-1. A synthetic peptide modeled after the sequence displayed by one of these phage, designated P3, was found to strongly inhibit the binding of laminin-1 to α6β1. This inhibitory effect of the P3 peptide seems to be specific for α6β1, since it did not affect the binding of fibronectin to integrin α5β1. A synthetic peptide with a scrambled P3 amino acid sequence barely inhibited the binding of laminin-1 to α6β1. When coated on a substratum after conjugation with bovine serum albumin, the P3 peptide was capable of promoting cell spreading in an α6β1-dependent manner, although the peptide with the scrambled sequence showed activity similar to that of a control peptide. These results taken together indicate that the P3 peptide is a novel ligand for integrin α6β1 with potent cell spreading activity. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence cell adhesion nationallicence integrin α6β1 nationallicence laminin-1 nationallicence phage display library nationallicence synthetic peptides nationallicence Murayama Ohoshi Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo , Izuni, Osaka 590-02 aut Nishida Hitoshi Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo , Izuni, Osaka 590-02 aut Sekiguchi Kiyotoshi Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo , Izuni, Osaka 590-02 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 445-451 0021-924X 120:2<445 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021431 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021431 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Murayama Ohoshi Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo , Izuni, Osaka 590-02 aut NATIONALLICENCE 700 1- Nishida Hitoshi Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo , Izuni, Osaka 590-02 aut NATIONALLICENCE 700 1- Sekiguchi Kiyotoshi Research Institute, Osaka Medical Center for Maternal and Child Health, 840 Murodo , Izuni, Osaka 590-02 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 445-451 0021-924X 120:2<445 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford