caa a22 4500 39755687X CHVBK 20180308164805.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021408 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021408 Structure and Expression of a Murine Homologue of sky Receptor Tyrosine Kinase Gene [Elektronische Daten] [Masato Sasaki, Jumpei Enami] To identify new receptor tyrosine kinases (RTKs), we screened cDNAs from mouse mammary tumor cells and mouse brain. A homology search of the complete cDNA sequences obtained showed that one cDNA was a murine homologue of recently reported human sky [Ohashi, K.et al. (1994) Oncogene 9,699-705]. Another cDNA obtained was also related to sky but had a 5′ upstream sequence similar to brt [Fujimoto, J. and Yamamoto, T. (1994) Oncogene 9, 693-698]. Analysis of the 5′ region of the sky genomic DNA revealed that brt-type and sky-type sequences are encoded by thesky gene in different exons. The upstream region of the sky-type coding exon is highly GC-rich and contains potential recognition sites for the Sp1 trans-acting factor, but lacks TATA and CAAT boxes, features commonly found in promoters of other RTKs. To examine whether this upstream region functions as a promoter, we fused it with chloramphenicol acetyltransferase (CAT) gene and transfected the construct into COS-7 cells. The results of the CAT assay showed that the sky upstream region retains a significant promoter activity. Furthermore, primer extension analysis revealed that the transcription starts at -240 nt upstream from the sky translation initiation codon. These observations suggest that the brt- and sky-types of mRNA are transcribed from a single sky gene by an alternative promoter usage. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence brain nationallicence mammary tumor nationallicence promoter nationallicence receptor tyrosine kinase nationallicence sky nationallicence Sasaki Masato Research Laboratory, Zenyaku Kogyo Co., Ltd., Oizumi, Nerima-ku, Tokyo 178 aut Enami Jumpei Research Laboratory, Zenyaku Kogyo Co., Ltd., Oizumi, Nerima-ku, Tokyo 178 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 264-270 0021-924X 120:2<264 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021408 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021408 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sasaki Masato Research Laboratory, Zenyaku Kogyo Co., Ltd., Oizumi, Nerima-ku, Tokyo 178 aut NATIONALLICENCE 700 1- Enami Jumpei Research Laboratory, Zenyaku Kogyo Co., Ltd., Oizumi, Nerima-ku, Tokyo 178 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 264-270 0021-924X 120:2<264 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford