caa a22 4500 39755690X CHVBK 20180308164805.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021428 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021428 Molecular Cloning and Characterization of Human Non-Smooth Muscle Calponin [Elektronische Daten] [Hiroaki Masuda, Kazuhiko Tanaka, Michiko Takagi, Kinya Ohgami, Tetsuo Sakamaki, Nobuhiko Shibata, Katsuhito Takahashi] cDNA clones encoding a calponin isoform with 309 amino acids have been isolated from human heart. The deduced amino acid polypeptide (Mr 33,697) showed a neutral isoelectric point of 7.1. The mRNA, expressed in cultured smooth muscle cells as well as in fibroblasts, vascular endothelial cells, and keratinocytes, contains a 3′ untranslated region of 1.2 kilobases that includes an Alu repetitive sequence in the antisense direction. On the basis of the nucleotide sequence identity to an expressed sequence tag, HUM21ES93 [Cheng, J.-F., Boyartchuk, V., and Zhu, Y. (1994) Genomics 23,75-84], the human neutral calponin gene is assigned to chromosome 21q11.1. The amino acid sequence indicates that this protein is the human equivalent of mouse calponin-h2 (94.8% identity) [Strasser, P., Gimona, M., Moessler, H., Herzog, M., and Small, J.V. (1993) FEBS Lett 330, 13-18]. Three tandem repeats of 29 amino acids, a Vav-homologous region and an actin-binding sequence, originally identified in the basic calponin isoform, are conserved. There are two consensus phosphorylation sites for tyrosine kinase. An immunoreactive form of the neutral calponin appears to be localized with vinculin in the cell-to-cell junctions of cardiomyocytes. Mouse calponin-h2 is also expressed in both embryonic and adult heart. These results indicate that the human neutral calponin is a non-smooth muscle isoform, and may play a physiological role in cytoskeletal organization. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence actin-binding protein nationallicence Alu sequence nationallicence calponin nationallicence cardiac muscle nationallicence Vav protoncogene nationallicence Masuda Hiroaki Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut Tanaka Kazuhiko Department of Structural Biology Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut Takagi Michiko Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut Ohgami Kinya Pharmaceutical Research Laboratory, KIRIN Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut Sakamaki Tetsuo The Second Department of Internal Madicine, Gunma University School of Medicine 3-39-22 Showa-machi, Maebashr, Gunma 371 aut Shibata Nobuhiko Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut Takahashi Katsuhito Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 415-424 0021-924X 120:2<415 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021428 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021428 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Masuda Hiroaki Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut NATIONALLICENCE 700 1- Tanaka Kazuhiko Department of Structural Biology Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut NATIONALLICENCE 700 1- Takagi Michiko Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut NATIONALLICENCE 700 1- Ohgami Kinya Pharmaceutical Research Laboratory, KIRIN Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Sakamaki Tetsuo The Second Department of Internal Madicine, Gunma University School of Medicine 3-39-22 Showa-machi, Maebashr, Gunma 371 aut NATIONALLICENCE 700 1- Shibata Nobuhiko Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut NATIONALLICENCE 700 1- Takahashi Katsuhito Department of Medicine Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinan-ku, Osakq Osaka 537 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 415-424 0021-924X 120:2<415 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford