caa a22 4500 397556926 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021417 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021417 Identification and Partial Amino Acid Sequences of Seven S-RNases Associated with Self-Incompatibility of Japanese Pear, Pyrus pyrifolia Nakai [Elektronische Daten] [Takeahi Ishimizu, Yoshihiko Sato, Toshihiro Saito, Yumi Yoshimura, Shigemi Norioka, Tetsu Nakanishi, Fumio Sakiyama] S-allele-specific proteins (S-proteins) were separated and identified by two-dimensional (2D) gel electrophoresis from the style extract of 14 cultivars of Japaneae pear, Pyrus pyrifolia Nakai, which exhibits gametophytic self-incompatibility. These S-proteins were 30-32 kDa basic proteins with putative pIs of 9.6-10.1 and were distinct from the other proteins, which were common for all cultivars examined. Each S-protein was assigned to a given S-genotype baaed on electrophoretic mobility and the partial amino acid sequence. For S1 - to S7-proteins, five different N-terminal amino acid sequences sharing the YFQFTQQY sequence were determined. Since the same N-terminal amino acid sequences were found for both S1- and S7-proteins, and for S3- and S3-proteins, the two S-proteins of each pair were distinguished based on their electrophoretic behavior. The internal amino acid sequences of S2- and S4-proteins, determined for Achromobacter protease I (API) digests, revealed that these proteins are S2- and S4-RNaees, respectively. In the cultivar Nijisseiki, these two RNases were expressed from the white bud to mature flower stages when the cultivar acquires and enforces self-incompatibility. Osa-Nijiseeiki, a self-compatible mutant of Nijisseiki, prduced S2-RNase, but did not produce S4-RNase. The absence of S4-RNase was also observed in self-compatible off springs derived from Osa-Nijiseeiki. These results suggest that Japanese pear in the family Rosaceae possesses a gametophytic self-incompatibility system involving an S-RNase, and that a reduction or lack of expression of s4-RNase in the style is responsible for the self-compatibility of Osa-Nijisseiki. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Japanese pear nationallicence S-allele nationallicence self-incompatibility nationallicence S-RNase nationallicence two-dimensional gel electrophoresis nationallicence Ishimizu Takeahi Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Sato Yoshihiko Fruit Tree Research Station, Ministry of Agriculture Forestry and Fisheries, Tsukuba , Ibaraki 305 aut Saito Toshihiro Fruit Tree Research Station, Ministry of Agriculture Forestry and Fisheries, Tsukuba , Ibaraki 305 aut Yoshimura Yumi Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Norioka Shigemi Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Nakanishi Tetsu Department of Agriculture and Horticulture, Faculty of Agriculture, Kobe University Kobe, Hyogo 657 aut Sakiyama Fumio Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 326-334 0021-924X 120:2<326 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021417 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021417 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Ishimizu Takeahi Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Sato Yoshihiko Fruit Tree Research Station, Ministry of Agriculture Forestry and Fisheries, Tsukuba , Ibaraki 305 aut NATIONALLICENCE 700 1- Saito Toshihiro Fruit Tree Research Station, Ministry of Agriculture Forestry and Fisheries, Tsukuba , Ibaraki 305 aut NATIONALLICENCE 700 1- Yoshimura Yumi Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Norioka Shigemi Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Nakanishi Tetsu Department of Agriculture and Horticulture, Faculty of Agriculture, Kobe University Kobe, Hyogo 657 aut NATIONALLICENCE 700 1- Sakiyama Fumio Divison of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 326-334 0021-924X 120:2<326 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford