caa a22 4500 397556942 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199608 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021418 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021418 Molecular Cloning and Nucleotide Sequences of cDNAs Encoding S-Allele Specific Stylar RNases in a Self-Incompatible Cultivar and Its Self-compatible Mutant of Japanese Pear, Pyrus pyrifolia Nakail [Elektronische Daten] [Naoko Norioka, Shigemi Norioka, Yoshimi Ohnishi, Takeshi Ishimizu, Chitose Oneyama, Tetsu Nakanishi, Fumio Sakiyama] The genes encoding three RNases were cloned from the style of a self-incompatible cultivar, Nijisseiki (S2S4), and its self-compatible mutant, Osa-Nijisseiki (S2S4sm, sm means stylar part mutant), of Japanese pear. For Nijisseiki, cDNAs coding for two S-RNases (S2-RNase and S4-RNase) and an RNase unrelated to self-incompatibility (non-S-RNase) were cloned from the stylar cDNA library. The cDNAs coding for S2-RNase, S4-RNase, and non-S-RNase include 678-, 684, and 681-bp open reading frames, respectively. Their deduced amino acid sequences were composed of signal peptides and mature RNases (201-203 residues) which were verified by partial amino acid sequencing. The primary structures of mature proteins revealed that these RNasea are of the RNase T2 type; only the two S-RNases have several potential N-glycosylation sites and 60% of their amino acid residues are identical, compared with 25% sequence identity with the non-S-RNase. Such a distinct difference in the primary structures between S-RNases and non-S-RNase has not previously been reported and may be a feature typical of S-RNases in the family Rosaceae. Similar experiments were performed for Osa-Nijisseiki. The cDNAs coding for S2-RNaae and non-S4-RNase were similarly cloned from the stylar cDNA library. However, the cDNA coding for S4-RNase was neither amplified by PCR nor cloned from the library, suggesting that the mutation of self-incompatible Nijisseiki to self-compatible Osa-Nijisseiki is due to a failure of expression of S4-RNase. These results lead to the idea that Osa-Nijisseiki is a variant of Nijisseiki in which the S4-allelic gene in the S-locus is exclusively mutated or deleted, causing severely impaired or suppressed expression of its gene product, S4-RNase,at the style. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence cloning nationallicence Japanese pear nationallicence nucleotide sequence nationallicence self-incompatibility nationallicence S-RNase nationallicence Norioka Naoko Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Norioka Shigemi Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Ohnishi Yoshimi Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Ishimizu Takeshi Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Oneyama Chitose Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut Nakanishi Tetsu Department of Agriculture and Horticulture, Faculty of Agriculture, Kobe University Kobe, Hyogo 657 aut Sakiyama Fumio Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 120/2(1996-08), 335-345 0021-924X 120:2<335 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021418 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021418 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Norioka Naoko Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Norioka Shigemi Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Ohnishi Yoshimi Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Ishimizu Takeshi Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Oneyama Chitose Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 700 1- Nakanishi Tetsu Department of Agriculture and Horticulture, Faculty of Agriculture, Kobe University Kobe, Hyogo 657 aut NATIONALLICENCE 700 1- Sakiyama Fumio Division of Protein Chemistry, Institute for Protein Research, Osaka University Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/2(1996-08), 335-345 0021-924X 120:2<335 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford