caa a22 4500 397556985 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021388 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021388 A Preliminary Description of the Crystal Structure of γ-Glutamyltranspeptidase from E. coli K-12 [Elektronische Daten] [Hiroaki Sakai, Noriyoshi Sakabe, Kyoyu Sasaki, Wataru Hashimoto, Hideyuki Suzuki, Hiromi Tachi, Hidehiko Kumagai, Kiwako Sakabe] The structure of GGT [EC 2.3.2.2] from E. coli K-12 was studied at 3 Å resolution by X-ray crystallography. Initial protein phases were calculated using two kinds of Pb2+ derivatives. The phases were refined by non-crystallographic 2-fold symmetry electron density averaging combined with solvent flattening and histogram matching. The GGT molecule has overall dimensions of 60×50×40 Å. There are two antiparallel β-pleated sheets consisting of 6 and β-strands. The two β-sheets form a wall-like structure. Twelve short α-helices were detected, of which the maximum length appears to be four helix turns. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence crystal structure analysis nationallicence density modification nationallicence γ-glutamyltranspeptidase nationallicence Sakai Hiroaki Department of Synchrotron Radiation Science, The Graduate University for Advanced Studies and Photon Factory, National Laboratory for High Energy Physics Oho, Tsukuba, Ibaraki 305 aut Sakabe Noriyoshi Institute of Applied Biochemistry and Center for Tsukuba Advanced Research Alliance, University of Tsukuba Tennoudai, Tsukuba, Ibaraki 305 aut Sasaki Kyoyu College of Medical Technology, Nagoya University Higashi-ku, Nagoya 461 aut Hashimoto Wataru Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut Suzuki Hideyuki Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut Tachi Hiromi Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut Kumagai Hidehiko Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut Sakabe Kiwako Department of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 26-28 0021-924X 120:1<26 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021388 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021388 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sakai Hiroaki Department of Synchrotron Radiation Science, The Graduate University for Advanced Studies and Photon Factory, National Laboratory for High Energy Physics Oho, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Sakabe Noriyoshi Institute of Applied Biochemistry and Center for Tsukuba Advanced Research Alliance, University of Tsukuba Tennoudai, Tsukuba, Ibaraki 305 aut NATIONALLICENCE 700 1- Sasaki Kyoyu College of Medical Technology, Nagoya University Higashi-ku, Nagoya 461 aut NATIONALLICENCE 700 1- Hashimoto Wataru Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Suzuki Hideyuki Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Tachi Hiromi Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Kumagai Hidehiko Department of Food Science and Technology, Faculty of Agriculture, Kyoto University Sakyo-ku, Kyoto 606-01 aut NATIONALLICENCE 700 1- Sakabe Kiwako Department of Chemistry, Faculty of Science, Nagoya University Chikusa-ku, Nagoya 464-01 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 26-28 0021-924X 120:1<26 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford