caa a22 4500 397557019 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021391 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021391 Application of Primary Hepatocytes from p53-Knockout Mice for Studies of Expression of Cyp3a [Elektronische Daten] [Miyuki Shimoji, Susumu Itoh, Kenji Toide, Kazuo Nakayama, Shinichi Aizawa, Tetsuya Kamataki] CYP3A rapidly disappears in primary hepatocytes, although the primary cells are suitable for studies of the regulation of CYP3A genes. In the present study, we found that Cyp3a mRNA could be expressed in the primary hepatocytes from p53-knockout mice for at least 2 weeks when the cells were cultured in the presence of dexamethasone. Propoxycoumarin O-depropylase activity, which is known to be mainly catalyzed by CYP3A, was maintained at a level of 50% of the initial activity even after 5 days of culture, and the activity correlated with the expression level of Cyp3a mRNA in the primary hepatocytes from p53-knockout mice. The cells remained morphologically intact during 4 weeks. These results suggest that hepatocytes from p53-knockout mice are a useful tool for studies of the expression of Cyp3a. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence Cyp3a nationallicence dexamethasone nationallicence hepatocytes nationallicence p53 nationallicence primary culture nationallicence Shimoji Miyuki Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut Itoh Susumu Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut Toide Kenji Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut Nakayama Kazuo Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut Aizawa Shinichi Laboratory of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine 2-2-1 Honjo, Kumamoto, Kumamoto 860 aut Kamataki Tetsuya Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 42-48 0021-924X 120:1<42 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021391 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021391 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Shimoji Miyuki Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Itoh Susumu Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Toide Kenji Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Nakayama Kazuo Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 700 1- Aizawa Shinichi Laboratory of Morphogenesis, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine 2-2-1 Honjo, Kumamoto, Kumamoto 860 aut NATIONALLICENCE 700 1- Kamataki Tetsuya Division of Drug Metabolism, Faculty of Pharmaceutical Sciences, Hokkaido University N12W6, Kita-ku, Sapporo, Hokkaido 060 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 42-48 0021-924X 120:1<42 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford