caa a22 4500 397557043 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021374 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021374 The 160k α1(IV) Chain, a Short Form of a Type IV Collagen Polypeptide, of Bovine Lens Capsule Retains the NC1 Domain [Elektronische Daten] [Masao Iwata, Yoshikazu Sado, Tasuku Sasaki, Yasutada Imamura, Yoshifumi Ninomiya, Toshihiko Hayashi] We recently reported that the bovine lens capsule contained a shorter α1(IV) chain (160k) as a major polypeptide in addition to the 180k α1(IV) chain [J. Biochem. (1995) 117, 1298-1304]. Two experiments were performed to examine whether or not the 160k polypeptide retained the carboxyl-terminal NC1 domain. On immunoblotting analysis with a monoclonal antibody (H11) raised against the NC1 domain of the human α1(IV) chain [position 1643-1650; near the carboxyl-terminal end of the human α1(IV) chain], the 180k and 160k polypeptides showed identical immunoreactivity, suggesting that the two chains had the same human α1(IV) collagen NC1 domain sequence. Another monoclonal antibody (H21) specific for the NC1 domain of human α2(IV) did not react with these polypeptides, but with the bands corresponding to 175k and 155k. The 160k polypeptide was selectively solubilized from bovine lens capsules, leaving the other major polypeptides, 180k and 175k, insoluble. The 160k polypeptide was separated by preparative electrophoresis. Bacterial collagenase digestion of the separated 160k polypeptide produced collagenase-resistant segments of about 29k and 30k in size based on globular standards. These sizes corresponded well with those of the NC1 domains of type IV collagen α chains (25-30k). The results indicated that the 160k polypeptide retained the carboxyl-terminal NC1 domain of the α1(IV) chain. In turn, the 20k polypeptide of the amino-terminal region or the 7S domain of 180k α1(IV) would have been excised to yield 160k α1(IV), assuming that the 160k α1(IV) chain is a processed form of the 180k α1(IV) one and not an alternatively spliced chain of the α1(IV) gene. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence α1(IV) collagen nationallicence basement membrane nationallicence bovine lens capsule nationallicence collagen IV nationallicence noncollagenous domain nationallicence Iwata Masao Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut Sado Yoshikazu Division of Immunology, Shigei Medical Research Institute Yamada, Okayama 701-02 aut Sasaki Tasuku Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut Imamura Yasutada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut Ninomiya Yoshifumi Department of Molecular Biology and Biochemistry, Okayama University Medical School, Shikata-cho Okayama 700 aut Hayashi Toshihiko Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 133-137 0021-924X 120:1<133 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021374 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021374 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Iwata Masao Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Sado Yoshikazu Division of Immunology, Shigei Medical Research Institute Yamada, Okayama 701-02 aut NATIONALLICENCE 700 1- Sasaki Tasuku Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Imamura Yasutada Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut NATIONALLICENCE 700 1- Ninomiya Yoshifumi Department of Molecular Biology and Biochemistry, Okayama University Medical School, Shikata-cho Okayama 700 aut NATIONALLICENCE 700 1- Hayashi Toshihiko Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo Meguro-ku, Tokyo 153 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 133-137 0021-924X 120:1<133 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford