caa a22 4500 397557051 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021390 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021390 Dephosphorylation of Cofilin in Parotid Acinar Cells [Elektronische Daten] [Taishin Takuma, Tokuro Ichida, Noriko Yokoyama, Shinri Tamura, Takashi Obinata] Cofilin is an actin-depolymerizing protein, whose depolymerizing activity is supposed to be regulated in part by phosphorylation and dephosphorylation. Thus, we studied the phosphorylation states of cofilin in rat parotid acinar cells during stimulation for amylase exocytosis. Isoproterenol and carbachol induced rapid and extensive dephosphorylation of cofilin; 60-70% dephosphorylation was clearly detectable within 1 min. Membrane-permeable cyclic AMP (CPS-cAMP), phorbol ester (PMA), and Ca ionophore A23187 mimicked the effect of isoproterenol and carbachol. Protein phosphatase inhibitors (calyculin A or FK506 plus cyclosporin A) did not block the dephosphorylation in response to isoproterenol or carbachol. Furthermore, calyculin A alone strongly dephosphorylated cofilin. Although no exogenous protein phosphatases tested dephosphorylated cofilin in the homogenate, the cofilin that was isolated by immunoprecipitation was clearly dephosphorylated by protein phosphatases 1, 2A, and 2C. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence amylase exocytosis nationallicence cofilin dephosphorylation nationallicence parotid gland nationallicence protein phosphatase nationallicence Takuma Taishin Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido Tobetsu, Hokkaido 061-02 aut Ichida Tokuro Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido Tobetsu, Hokkaido 061-02 aut Yokoyama Noriko Department of Biochemistry, University of Western Ontario London, Canada N6A 5C1 aut Tamura Shinri Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University Sendai 980 aut Obinata Takashi Department of Biology, Faculty of Science, Chiba University Chiba 260 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 35-41 0021-924X 120:1<35 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021390 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021390 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takuma Taishin Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido Tobetsu, Hokkaido 061-02 aut NATIONALLICENCE 700 1- Ichida Tokuro Department of Oral Biochemistry, School of Dentistry, Health Sciences University of Hokkaido Tobetsu, Hokkaido 061-02 aut NATIONALLICENCE 700 1- Yokoyama Noriko Department of Biochemistry, University of Western Ontario London, Canada N6A 5C1 aut NATIONALLICENCE 700 1- Tamura Shinri Department of Biochemistry, Institute of Development, Aging and Cancer, Tohoku University Sendai 980 aut NATIONALLICENCE 700 1- Obinata Takashi Department of Biology, Faculty of Science, Chiba University Chiba 260 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 35-41 0021-924X 120:1<35 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford