caa a22 4500 397557086 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021379 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021379 Autocrine Amplification of Type I Interferon Gene Expression Mediated by Interferon Stimulated Gene Factor 3 (ISGF3) [Elektronische Daten] [Mitsutoshi Yoneyama, Wakako Suhara, Yukiko Fukuhara, Mayumi Sato, Keiko Ozato, Takashi Fujita] Interferon regulatory factor (IRF)-1 and IRF-2 have been implicated for the virus-induced expression of the interferon-α and β (type I IFN) genes. However, recent gene disruption studies in mice suggested the presence of other factor(s) interacting with overlapping promoter elements. In the present paper, we describe the characterization of a DNA binding factor which is strongly induced after virus infection and recognizes these promoter elements. After extensive purification, the factor was revealed to be identical to IFN-stimulated gene factor 3 (ISGF3), a transcription factor complex activated by IFN treatment. ISGF3 binds to the promoter element of IFN-β positive regulatory domain I (PRDI), with significantly higher affinity than IRF-1, 2, and mutational analysis of PRDI showed that the gene expression and binding of ISGF3, but not of IRF-1, 2, are highly correlated. Furthermore, our functional analysis involving a dominant negative inhibitor for ISGF3 activation and an anti-IFN neutralizing antibody clearly demonstrated the presence of a positive feedback pathway for type I IFN genes mediated by ISGF3. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence gene regulation nationallicence IRF nationallicence ISGF3 nationallicence type I interferon nationallicence viral induction nationallicence Yoneyama Mitsutoshi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Suhara Wakako Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Fukuhara Yukiko Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Sato Mayumi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut Ozato Keiko Department of Health and Human Services, National Institute of Child Health and Human Development Bethesda, Maryland 20892, USA aut Fujita Takashi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 160-169 0021-924X 120:1<160 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021379 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021379 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yoneyama Mitsutoshi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Suhara Wakako Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Fukuhara Yukiko Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Sato Mayumi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Ozato Keiko Department of Health and Human Services, National Institute of Child Health and Human Development Bethesda, Maryland 20892, USA aut NATIONALLICENCE 700 1- Fujita Takashi Department of Tumor Cell Biology, The Tokyo Metropolitan Institute of Medical Science 3-18-22 Honkomagome, Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 160-169 0021-924X 120:1<160 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford