caa a22 4500 397557108 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021392 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021392 Concomitant Transcriptional Activation of Nitric Oxide Synthase and Heme Oxygenase Genes during Nitric Oxide-Mediated Macrophage Cytostasis [Elektronische Daten] [Shun-ichi Kurata, Midori Matsumoto, Uki Yamashita] During in vitro activation of mouse peritoneal macrophages with interferon-γ (IFN-γ) and lipopolysaccharide (LPS), their synthesis of peroxynitrite and their cytostatic activity against mouse lymphocytic leukemia (L1210) cells were examined. The activation of the genes for nitric oxide synthase (iNOS) and heme oxygenase (HO-1) was also determined during the activation of the macrophages. Results showed that activation of peroxynitrite synthesis in macrophages was accompanied by the transcriptional activation of iNOS and HO-1 genes. Both genes seem to be activated simultaneously upon activation of the macrophages. Simultaneous activation of iNOS and HO-1 genes may be important because degradation of heme by HO-1 is one of the most important reaction that produces CO in higher organisms, and nitric oxide (NO) and carbon monoxide (CO) can react with heme-containing guanylate cyclase. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence carbon monoxide nationallicence cytostasis nationallicence heme oxygenase nationallicence nitric oxide nationallicence nitric oxide synthase nationallicence Kurata Shun-ichi Department of Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University Bunkyo-ku, Tokyo 113 aut Matsumoto Midori Department of Life Science and Technology, Tokyo Institute of Technology Kanagawa 226 aut Yamashita Uki Department of Immunology and Patasitology, Hiroshima University School of Medicine Hiroshima 734 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 49-52 0021-924X 120:1<49 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021392 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021392 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kurata Shun-ichi Department of Biochemical Genetics, Medical Research Institute, Tokyo Medical and Dental University Bunkyo-ku, Tokyo 113 aut NATIONALLICENCE 700 1- Matsumoto Midori Department of Life Science and Technology, Tokyo Institute of Technology Kanagawa 226 aut NATIONALLICENCE 700 1- Yamashita Uki Department of Immunology and Patasitology, Hiroshima University School of Medicine Hiroshima 734 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 49-52 0021-924X 120:1<49 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford