caa a22 4500 397557116 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021387 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021387 Identification of a Ribosomal Frameshift in Leishmania RNA Virus 1-4 [Elektronische Daten] [Sang Eun Lee, Jae Myoung Suh, Scott Scheffter, Jean L. Patterson, In Kwon Chung] Double-stranded Leishmania RNA virus 1-4 (LRV 1-4) has at least four open reading frames (ORFs). The two small ORFs located near its 5′ terminus, ORF1 and ORFx, could encode 34- and 60-amino acid polypeptides, respectively. ORF2 encodes an 82-kDa major capsid protein, and 0RF3 encodes a 98-kDa polypeptide which contains the consensus sequence for RNA-dependent RNA polymerases of plus-strand and double-stranded RNA viruses. The complete sequence of LRV 1-4 shows that 0RF2 and ORF3 overlap by 71 nucleotides, and that 0RF3 lacks a potential translation initiation site, suggesting that the viral polymerase may be synthesized as a 180-kDa fusion protein with the virus capsid. In this report, we present evidence for the synthesis of a fusion protein through a ribosomal frameshift. In vitro-translation experiments and immunostudies involving antiserum against the viral capsid protein demonstrated that the overlapping 71 nucleotides of ORF2 and 0RF3 are contained in a region which promotes translational frameshifting. Computer analysis of the putative frameshift region revealed a potential pseudoknot structure located within the overlapping 71 nucleotide sequence. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence capsid protein nationallicence Leishmania RNA virus nationallicence pseudoknot structure nationallicence ribosomal frameshift nationallicence RNA-dependent RNA polymerases nationallicence Lee Sang Eun Department of Biology, and Bioproducts Research Center, Yonsei University Seoul, 120-749, Korea aut Suh Jae Myoung Department of Biology, and Bioproducts Research Center, Yonsei University Seoul, 120-749, Korea aut Scheffter Scott Division of Infectious Diseases, Children's Hospital, and Department of Microbiology and Molecular Genetics, Harvard Medical School Boston, Massachusetts 02115, USA aut Patterson Jean L. Division of Infectious Diseases, Children's Hospital, and Department of Microbiology and Molecular Genetics, Harvard Medical School Boston, Massachusetts 02115, USA aut Chung In Kwon Department of Biology, and Bioproducts Research Center, Yonsei University Seoul, 120-749, Korea aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 22-25 0021-924X 120:1<22 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021387 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021387 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Lee Sang Eun Department of Biology, and Bioproducts Research Center, Yonsei University Seoul, 120-749, Korea aut NATIONALLICENCE 700 1- Suh Jae Myoung Department of Biology, and Bioproducts Research Center, Yonsei University Seoul, 120-749, Korea aut NATIONALLICENCE 700 1- Scheffter Scott Division of Infectious Diseases, Children's Hospital, and Department of Microbiology and Molecular Genetics, Harvard Medical School Boston, Massachusetts 02115, USA aut NATIONALLICENCE 700 1- Patterson Jean L. Division of Infectious Diseases, Children's Hospital, and Department of Microbiology and Molecular Genetics, Harvard Medical School Boston, Massachusetts 02115, USA aut NATIONALLICENCE 700 1- Chung In Kwon Department of Biology, and Bioproducts Research Center, Yonsei University Seoul, 120-749, Korea aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 22-25 0021-924X 120:1<22 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford