caa a22 4500 397557124 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021382 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021382 The 5′-Flanking Region of the Human Smooth Muscle Cell Calponin Gene Contains a cis-Acting Domain for Interaction with a Methylated DNA-Binding Transcription Repressor [Elektronische Daten] [Katsuhito Takahashi, Tetsushi Tazunoki, Tsutomu Okada, Kinya Ohgami, Takeshi Miwa, Akiko Miki, Nobuhiko Shibata] The human smooth muscle cell (SMC) calponin gene, which is composed of seven exons and six introns, spanning an approximately 11.2 kilobase (kb) genomic DNA, has been isolated and characterized by sequence analysis. As determined by primer extension mapping and rapid amplification of mRNA transcripts, a major transcription start site of the calponin gene is located at 101 base-pairs (bp) upstream of the ATG start codon. A striking feature of the 5′-flanking, 5′-untranslated, and amino-terminal protein coding regions is the presence of an Alu repetitive sequence and a stretch of DNA sequence identified using a methyl CpG-binding protein affinity column [Cross, S.H., Charlton, J.A., Nan, X., and Bird, A.P. (1994) Nature Genet. 6, 236-244]. The results of this study provide a new insight into the molecular mechanism underlying regulation of SMC-lineage specific gene expression. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Rapid Communications nationallicence actin-binding protein nationallicence Alu repetitive sequence nationallicence calponin nationallicence CpG island nationallicence DNA methylation nationallicence smooth muscle-specific gene nationallicence Takahashi Katsuhito Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka 537 aut Tazunoki Tetsushi Pharmaceutical Research Laboratory, Kirm Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut Okada Tsutomu Pharmaceutical Research Laboratory, Kirm Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut Ohgami Kinya Pharmaceutical Research Laboratory, Kirm Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut Miwa Takeshi Genome Information Research Center Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Miki Akiko Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka 537 aut Shibata Nobuhiko Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka 537 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 18-21 0021-924X 120:1<18 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021382 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021382 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Takahashi Katsuhito Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka 537 aut NATIONALLICENCE 700 1- Tazunoki Tetsushi Pharmaceutical Research Laboratory, Kirm Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Okada Tsutomu Pharmaceutical Research Laboratory, Kirm Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Ohgami Kinya Pharmaceutical Research Laboratory, Kirm Brewery Co., Ltd. 1-2-2 Souja-machi, Maebashi, Gunma 371 aut NATIONALLICENCE 700 1- Miwa Takeshi Genome Information Research Center Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Miki Akiko Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka 537 aut NATIONALLICENCE 700 1- Shibata Nobuhiko Department of Medicine, Molecular Biology Unit, Osaka Medical Center for Cancer and Cardiovascular Diseases 1-3-3 Nakamichi, Higashinari-ku, Osaka, Osaka 537 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 18-21 0021-924X 120:1<18 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford