caa a22 4500 397557132 CHVBK 20180308164805.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021396 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021396 Characterization of Seed Oil Bodies and Their Surface Oleosin Isoforms from Rice Embryos [Elektronische Daten] [Rachel L.C. Chuang, Jeff C.F. Chen, Jessie Chu, Jason T.C. Tzen] Plant seeds store triacylglycerols in discrete organelles called oil bodies. An oil body stores a matrix of triacylglycerols surrounded by phospholipids and alkaline proteins termed oleosins. Oil bodies in rice seeds are present in embryos and aleurone layers. They do not coalesce in crowded environments, as observed on electron microscopy. The detected isoelectric point of purified rice oil bodies is pH 6.2. This implies that rice oil bodies possess a negatively charged surface at neutral pH. The suspension of rice oil bodies in pH 6.5 buffer induces aggregation. Presumably, the negatively charged surface causes electrostatic repulsion that maintains rice oil bodies as discrete organelles. Rice oil bodies lose their integrity on trypsin treatment. Undoubtedly, oleosins play an important role in the stability of oil bodies. There are two oleosin isoforms in rice oil bodies. Antibodies raised against these two homologous isoforms do not cross-recognize each other. Both isoforms are restricted to oil bodies, as detected on immuno-assaying. Partial amino acid sequences of these two isoforms were obtained, and compared with the deduced sequences of two maize and two rice oleosin genes. The comparison confirmed that the two major proteins in rice oil bodies are the two oleosin isoforms. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence isoform nationallicence oil body nationallicence oleosin nationallicence rice nationallicence structure-function nationallicence Chuang Rachel L.C. Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut Chen Jeff C.F. Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut Chu Jessie Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut Tzen Jason T.C. Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 74-81 0021-924X 120:1<74 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021396 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021396 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Chuang Rachel L.C. Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut NATIONALLICENCE 700 1- Chen Jeff C.F. Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut NATIONALLICENCE 700 1- Chu Jessie Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut NATIONALLICENCE 700 1- Tzen Jason T.C. Graduate Institute of Agricultural Biotechnology, National Chung-Hsing University, Taichung, Taiwan 40227 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 74-81 0021-924X 120:1<74 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford