caa a22 4500 39755723X CHVBK 20180308164806.0 cr unu---uuuuu 161202e199607 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021378 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021378 Human Homolog of Drosophila Heterochromatin-Associated Protein 1 (HP1) Is a DNA-Binding Protein Which Possesses a DNA-Binding Motif with Weak Similarity to That of Human Centromere Protein C (CENP-C) [Elektronische Daten] [Kenji Sugimoto, Taku Yamada, Yoshinao Muro, Michio Himeno] Heterochromatin-associated protein 1 (HP1) is a nonhistone chromosomal component tightly associated with the pericentromeric heterochromatic region of fruit fly, mouse, and human throughout the cell cycle. Drosophila HP1 has been shown to be involved in position effect variegation and to be required for the correct chromosome segregation in vivo, while the biological activity of human homolog (HP1hsa) has not yet been characterized. We previously reported that human CENP-B and CENP-C, two major centromere heterochromatin autoantigens often recognized by autosera in scleroderma patients, possess DNA-binding activity in vitro. Here, we show that human HP1 which is also an autoantigen targeted by some types of anticentromere autosera, is a DNA-binding protein. Human HP1 was expressed as a GST-fusion in Escherichia coli and purified with glutathione-Se pharose. The DNA-binding activity of the recombinant HP1 was demonstrated by gel mobility shift assay and South-Western-type blotting. The minimum DNA-binding region was further limited to the internal 64-amino acid stretch that is less-conserved between human and fruit fly but retains a helix-enriched motif with weak similarity to CENP-C. This suggests that HP1 is involved in the pericentromeric heterochromatin formation by directly associating with genomic DNA. © 1996 BY THE JOURNAL OF BIOCHEMISTRY Regular Papers nationallicence autoantibody nationallicence centromere nationallicence DNA binding nationallicence heterochromatin nationallicence HP1 nationallicence Sugimoto Kenji Laboratory of Applied Molecular Biology, Department of Applied Biochemistry, University of Osaka Prefecture 1-1 Gakuen-cho, Sakai Osaka 593 aut Yamada Taku Laboratory of Applied Molecular Biology, Department of Applied Biochemistry, University of Osaka Prefecture 1-1 Gakuen-cho, Sakai Osaka 593 aut Muro Yoshinao Department of Dermatology, Nagoya University School of Medicine and all concurrently Division of Marginal Science, Research Institute for Advanced Science and Technology, University of Osaka Prefecture 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut Himeno Michio Laboratory of Applied Molecular Biology, Department of Applied Biochemistry, University of Osaka Prefecture 1-1 Gakuen-cho, Sakai Osaka 593 aut The Journal of Biochemistry Oxford University Press 120/1(1996-07), 153-159 0021-924X 120:1<153 1996 120 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021378 text/html Onlinezugriff via DOI 1 other jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021378 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Sugimoto Kenji Laboratory of Applied Molecular Biology, Department of Applied Biochemistry, University of Osaka Prefecture 1-1 Gakuen-cho, Sakai Osaka 593 aut NATIONALLICENCE 700 1- Yamada Taku Laboratory of Applied Molecular Biology, Department of Applied Biochemistry, University of Osaka Prefecture 1-1 Gakuen-cho, Sakai Osaka 593 aut NATIONALLICENCE 700 1- Muro Yoshinao Department of Dermatology, Nagoya University School of Medicine and all concurrently Division of Marginal Science, Research Institute for Advanced Science and Technology, University of Osaka Prefecture 65 Tsurumai-cho, Showa-ku, Nagoya 466 aut NATIONALLICENCE 700 1- Himeno Michio Laboratory of Applied Molecular Biology, Department of Applied Biochemistry, University of Osaka Prefecture 1-1 Gakuen-cho, Sakai Osaka 593 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 120/1(1996-07), 153-159 0021-924X 120:1<153 1996 120 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford