caa a22 4500 397557353 CHVBK 20180308164806.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021313 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021313 The Protective Role of Glutathione Peroxidase in Apoptosis Induced by Reactive Oxygen Species [Elektronische Daten] [Yoshiro Kayanoki, Junichi Fujii, Kazi Nazrul Islam, Keiichiro Suzuki, Sumio Kawata, Yuji Matsuzawa, Naoyuki Taniguchi] Selenium-dependent glutathione peroxidase (GPx) plays a protective role in oxidative stress-induced apoptosis. In this study, we demonstrated that MDBK cells, a bovine renal epithelial cell line, exhibited internucleosomal DNA fragmentation characteristic of apoptotic cell death under selenium-deficient conditions with lower doses of hydrogen peroxide (H2O2) than under selenium-supplemented ones. This was due to a decreased amount of GPx in the cells under selenium-deficient conditions, because other antioxidative enzyme activities were not affected by the selenium supplementation. Cumene hydroperox-ide also induced DNA fragmentation in selenium-deficient cells but no ladder formation was observed. Flow cytometric analysis showed that selenium-deficient cells were less capable of scavenging intracellular peroxides after exposure to exogenous H2O2 than selenium-supplemented ones. In contrast, there was no difference in viability between selenium-supplemented and non-supplemented cells in cell survival after exposure to menadione,which activates the electron transport system and increases intracellular superoxide radicals. Clofibrate, a peroxisomal proliferator and an inducer of catalase (CAT), partially protected both Se-deficient and Se-supplemented cells from exogenous H2O2. We concluded that selenium-deficient cells were more easily brought to apoptotic cell death by peroxides, but not by superoxide radicals, than selenium-supplemented ones and that CAT could compensate for the depletion of GPx to a certain degree by scavenging H2O2. © by the Journal of Biochemistry Regular Papers nationallicence apoptosis nationallicence catalase nationallicence glutathione peroxidase nationallicence hydrogen peroxide nationallicence selenium deficiency nationallicence Kayanoki Yoshiro Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut Fujii Junichi Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut Islam Kazi Nazrul Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut Suzuki Keiichiro Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut Kawata Sumio Second Department of Internal Medicine, Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut Matsuzawa Yuji Second Department of Internal Medicine, Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut Taniguchi Naoyuki Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 817-822 0021-924X 119:4<817 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021313 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021313 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Kayanoki Yoshiro Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Fujii Junichi Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Islam Kazi Nazrul Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Suzuki Keiichiro Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Kawata Sumio Second Department of Internal Medicine, Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Matsuzawa Yuji Second Department of Internal Medicine, Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Taniguchi Naoyuki Department of Biochemistry Osaka University Medical School 2-2 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 817-822 0021-924X 119:4<817 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford