caa a22 4500 397557361 CHVBK 20180308164806.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021302 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021302 Analysis of Bile Acids in Urine Specimens from Healthy Humans: Determination of Several Bile Acids with β-Hydroxyl and Carbonyl Groups [Elektronische Daten] [Nobuo Yamaga, Jun Ikebuchi, Hiromi Kohara, Yoshio Ogura, Kazuo Yamada] Urinary bile acids of 39 healthy male undergraduates were analyzed by capillary gas chromatography and capillary gas chromatography-mass spectrometry. 3α-Hydroxy-12-oxo-5β-cholanoic acid, 3α,12β-dihydroxy-5β-cholanoic acid, 3β,7α-dihydroxy-5β-cholan-oic acid, and 3α,7α,12β-trihydroxy-5β-cholanoic acid, in addition to known bile acids, were identified and then quantified. The major part of the urinary bile acids was occupied by secondary bile acids. Every 7β-hydroxylated bile acid species was found in more than 80% of the subjects. The bile acid detected in the largest amount was 3α-hydroxy-12-oxo-5β-cholanoic acid. The metabolites of cholic acid were quantitatively more predominant than those of chenodeoxycholic acid. These results indicate that bile acids with β-hydroxyl and carbonyl groups at the C-3,7 and/or 12 positions are usual bile acids usually found in the urine of healthy humans. It is concluded that the occurrence of these bile acids is an effect of the intestinal bacterial flora and living conditions. © by the Journal of Biochemistry Regular Papers nationallicence 3α nationallicence 12β-dihydroxy-5β-cholanoic acid nationallicence healthy humans nationallicence 3α-hydroxy-12-oxo-5β-cholanoic acid nationallicence 3α nationallicence 7α nationallicence 2β-trihydroxy-5β-cholanoic acid nationallicence urine specimens nationallicence Yamaga Nobuo Department of Biochemistry,Faculty of Medicine, Tottori University Yonago, Tottori 683 aut Ikebuchi Jun Department of Legal Medicine, Faculty of Medicine, Tottori University Yonago, Tottori 683 aut Kohara Hiromi Department of Pathological Biochemistry, Faculty of Medicine, Tottori University Yonago, Tottori 683 aut Ogura Yoshio Department of Biochemistry,Faculty of Medicine, Tottori University Yonago, Tottori 683 aut Yamada Kazuo Department of Biochemistry,Faculty of Medicine, Tottori University Yonago, Tottori 683 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 725-730 0021-924X 119:4<725 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021302 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021302 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Yamaga Nobuo Department of Biochemistry,Faculty of Medicine, Tottori University Yonago, Tottori 683 aut NATIONALLICENCE 700 1- Ikebuchi Jun Department of Legal Medicine, Faculty of Medicine, Tottori University Yonago, Tottori 683 aut NATIONALLICENCE 700 1- Kohara Hiromi Department of Pathological Biochemistry, Faculty of Medicine, Tottori University Yonago, Tottori 683 aut NATIONALLICENCE 700 1- Ogura Yoshio Department of Biochemistry,Faculty of Medicine, Tottori University Yonago, Tottori 683 aut NATIONALLICENCE 700 1- Yamada Kazuo Department of Biochemistry,Faculty of Medicine, Tottori University Yonago, Tottori 683 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 725-730 0021-924X 119:4<725 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford