caa a22 4500 39755737X CHVBK 20180308164806.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021300 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021300 Partial Purification and Characterization of a Novel Soybean Protease Which is inhibited by Kunitz and Bowman-Birk Trypsin Inhibitors [Elektronische Daten] [Shimpei Morita, Masami Fukase, Kumiko Hoshino, Yoichi Fukuda, Masami Yamaguchi, Yuhei Morita] A novel serine protease has been partially purified from dry seeds of the soybean (Glycine max;) cultivar Keburi by cryoprecipitation at pH 6.4, fractional precipitation with ammonium sulfate, and a series of column chromatographic procedures on DEAE-Sepharose, SP-Sepharose, and Arginine-Sepharose 4B. Some properties of the purified enzyme were studied. The protease hydrolyzed the native storage globulins of soybean seeds, such as the α subunit of β-conglycinin, at a pair of arginine residues, Argl26-Argl27. The proteolysis of the α subunit in the purified α2β molecule of β-conglycinin apparently followed first order kinetics. The enzyme was inhibited by both soybean Kunitz trypsin inhibitor and Bowman-Birk proteinase inhibitor in a competitive manner. Moreover, the enzyme could catalyze the specific proteolysis of the A3 polypeptide of the purified G5 glycinin at the Arg99-Glyl00 linkage, or the carboxyl side of the Arg98-Arg99 paired basic residues. © by the Journal of Biochemistry Regular Papers nationallicence β-conglycinin nationallicence glycinin nationallicence serine protease nationallicence soybean nationallicence trypsin inhibitor nationallicence Morita Shimpei Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut Fukase Masami Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut Hoshino Kumiko Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut Fukuda Yoichi Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut Yamaguchi Masami Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut Morita Yuhei Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 711-718 0021-924X 119:4<711 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021300 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021300 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Morita Shimpei Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut NATIONALLICENCE 700 1- Fukase Masami Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut NATIONALLICENCE 700 1- Hoshino Kumiko Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut NATIONALLICENCE 700 1- Fukuda Yoichi Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut NATIONALLICENCE 700 1- Yamaguchi Masami Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut NATIONALLICENCE 700 1- Morita Yuhei Central Research Institute, Fuji Oil Co, 4-3 Kinunodai, Yawara-mura, Tsukuba-gun, Ibaruki 300-24 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 711-718 0021-924X 119:4<711 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford