caa a22 4500 397557396 CHVBK 20180308164806.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021303 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021303 Pore Formation on Proliferating Yeast Saccharomyces cerevisiae Cell Buds by HM-1 Killer Toxin [Elektronische Daten] [Tadazumi Komiyama, Tatsuo Ohta, Hiroshi Urakami, Yasuhiko Shiratori, Tsuyoshi Takasuka, Misako Satoh, Takahide Watanabe, Yasuhiro Furuichi] The cytocidal effect of HM-1 produced by Hansenula mrahii on yeast Saccharomyces cerevisiae; cells was studied. The HM-1 strongly inhibited the growth ofS. cerevisiae; cells at a low concentration (IC50;; 2.1 × 10−8;M) by reducing the number of viable cells. The killer action of HM-1 was most efficient when cells were actively proliferating. Cells in a resting state were resistant, but they became HM-1-sensitive after about 90 min of culturing at 30°C, concomitantly with the increment of budding index. In association with the reduction of viable cell number, ultraviolet light-absorbing cellular components were discharged from sensitive cells. HM-1 molecules appear to bind to susceptible cells rather loosely since cells incubated with HM-1 were able to proliferate after having been washed. By phase-contrast light microscopy and scanning electron microscopy, discharge of cell material was observed at the budding portions of HM-1-treated cells. Addition of sorbitol to make the culture medium isotonic partially reduced the cell death induced by HM-1. These results suggest that HM-1 acts on the budding region of proliferating yeast cells, resulting in pore formation, leakage of cell material and eventual cell death. © by the Journal of Biochemistry Regular Papers nationallicence antifungal drug nationallicence killer toxin nationallicence β-glucan synthase nationallicence budding nationallicence cell wall nationallicence yeast proliferation nationallicence Komiyama Tadazumi Department of Biochemistry, Niigata College of Pharmacy 5-13-2 Kamishinei-cho, Niigata 950-21 aut Ohta Tatsuo Department of Biochemistry, Niigata College of Pharmacy 5-13-2 Kamishinei-cho, Niigata 950-21 aut Urakami Hiroshi Department of Biochemistry, Niigata College of Pharmacy 5-13-2 Kamishinei-cho, Niigata 950-21 aut Shiratori Yasuhiko Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut Takasuka Tsuyoshi Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut Satoh Misako Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut Watanabe Takahide Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut Furuichi Yasuhiro AGENE Research Institute Kajiwara, Kamakura 247 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 731-736 0021-924X 119:4<731 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021303 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021303 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Komiyama Tadazumi Department of Biochemistry, Niigata College of Pharmacy 5-13-2 Kamishinei-cho, Niigata 950-21 aut NATIONALLICENCE 700 1- Ohta Tatsuo Department of Biochemistry, Niigata College of Pharmacy 5-13-2 Kamishinei-cho, Niigata 950-21 aut NATIONALLICENCE 700 1- Urakami Hiroshi Department of Biochemistry, Niigata College of Pharmacy 5-13-2 Kamishinei-cho, Niigata 950-21 aut NATIONALLICENCE 700 1- Shiratori Yasuhiko Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut NATIONALLICENCE 700 1- Takasuka Tsuyoshi Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut NATIONALLICENCE 700 1- Satoh Misako Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut NATIONALLICENCE 700 1- Watanabe Takahide Department of Molecular Genetics, Nippon Roche Research Center Kajiwara, Kamakura 247 aut NATIONALLICENCE 700 1- Furuichi Yasuhiro AGENE Research Institute Kajiwara, Kamakura 247 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 731-736 0021-924X 119:4<731 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford