caa a22 4500 397557418 CHVBK 20180308164806.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021305 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021305 A Catalogue of Genes in Mouse Embryonal Carcinoma F9 Cells Identified with Expressed Sequence Tags [Elektronische Daten] [Seiji Nishiguchi, Rui Sakuma, Midori Nomura, Zhihua Zou, Juree Jearanaisilavong, Tadashi Joh, Teruo Yasunaga, Kazuori Shimada] We used expressed sequence tags (ESTs) to identify genes expressed in mouse embryonal carcinoma F9 cells and prepared 2132 ESTs from undifferentiated F9 cDNA libraries: 1026 were prepared after randomly selecting clones from one of the libraries and the remaining 1106 ESTs were prepared after classifying 2896 clones of the libraries into four classes, according to the levels and patterns of expression. Among the former 1026 ESTs, 797 (78%) matched known genes, 61 (6%) matched database sequences of uncharacterized cDNAs, and 168 (16%) represented novel genes. The ESTs matching known genes were catalogued according to putative structural and cellular functions. As many as 53% were related to transcription and translation, and 19% were related to energy metabolism, including transcripts of mitochondrial DNA. These percentages were significantly higher in F9 cells than in the human heart and brain, and a human liver cell line, HepG2. We found that approximately 7% of the ESTs corresponding to low-abundance mRNAs are either related to retinoic acid-regulated genes or mammalian development-and/or differentiation-related genes. Cataloguing of the genes expressed in the F9 cells paves the way for isolating genes involved in early mammalian development. © by the Journal of Biochemistry Regular Papers nationallicence embryonal carcinoma nationallicence expressed sequence tags nationallicence F9 nationallicence low-abundance mRNAs nationallicence Nishiguchi Seiji Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Sakuma Rui Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Nomura Midori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Zou Zhihua Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Jearanaisilavong Juree Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Joh Tadashi Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut Yasunaga Teruo Genome Information Research Center, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut Shimada Kazuori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 749-767 0021-924X 119:4<749 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021305 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021305 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Nishiguchi Seiji Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Sakuma Rui Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Nomura Midori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Zou Zhihua Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Jearanaisilavong Juree Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Joh Tadashi Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Yasunaga Teruo Genome Information Research Center, Osaka University 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 700 1- Shimada Kazuori Department of Medical Genetics, Division of Molecular Biomedicine, Research Institute for Microbial Diseases Osaka University, 3-1 Yamadaoka, Suita, Osaka 565 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 749-767 0021-924X 119:4<749 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford