caa a22 4500 397557442 CHVBK 20180308164806.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021286 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021286 Coconut Oil Affects Lipoprotein Composition and Structure of Neonatal Chicks [Elektronische Daten] [Mercedes Castillo, José H Hortal, Eduardo García-Fuentes, María F. Zafra, Eduardo García-Peregrín] Supplementation of 10 or 20% coconut oil in the diet for 1-2 weeks produced a significant hypercholesterolemia in neonatal chicks. Plasma triacylglycerol concentration significantly increased after the addition of 20% coconut oil for 2 weeks. These results show that newborn chicks are more sensitive to saturated fatty acids from coconut oil than adult animals. The effects of this saturated fat on lipoprotein composition were studied for the first 1-2 weeks of neonatal chick life. Coconut oil supplementation in the diet (20-) for 2 weeks increased cholesterol concentration in all the lipoprotein fractions, while 10- coconut oil only increased cholesterol in low-density and very-low-density lipoproteins, an increase that was significant after 1 week of treatment. Similar results were obtained for triacylglycerol concentration after 2 weeks of treatment. Changes in phospholipid and total protein levels were less profound. Coconut oil decreased low-density and very-low-density lipoprotein fluidity, measured as total cholesterol/phospholipid ratio. Changes in esterified cholesterol/phospholipid and triacylglycerol/phospholipid ratios suggest that coconut oil affects the distribution of lipid components in the core of very-low-density particles. Likewise, the esterified cholesterol/triacylglycerol ratio was clearly increased in the low-density, and especially in the very-low-density, fraction after the first week of coconut oil feeding. Our results show that neonatal chick provides a suitable model in which to study the role of very-low-density lipoproteins in atherogenesis and the rapid response to saturated fatty acids with 12-14 carbons. © by the Journal of Biochemistry Regular Papers nationallicence atherogenesis nationallicence coconut oil nationallicence hypercholesterolemia nationallicence lipoprotein nationallicence neonatal chick nationallicence Castillo Mercedes Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut Hortal José H. Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut García-Fuentes Eduardo Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut Zafra María F. Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut García-Peregrín Eduardo Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 610-616 0021-924X 119:4<610 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021286 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021286 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Castillo Mercedes Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut NATIONALLICENCE 700 1- Hortal José H. Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut NATIONALLICENCE 700 1- García-Fuentes Eduardo Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut NATIONALLICENCE 700 1- Zafra María F. Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut NATIONALLICENCE 700 1- García-Peregrín Eduardo Department of Biochemistry and Molecular Biology, University of Granada, 18071 Granada aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 610-616 0021-924X 119:4<610 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford