caa a22 4500 397557477 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021311 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021311 Formation of a Triple-Stranded DNA between d(GGA: TCC) Repeats and d(GGA) Repeat Oligonucleotides [Elektronische Daten] [Yukio Mishima, Takeshi Suda, Ryo Kominami] Incubation of the 162-bp duplex containing d(GGA:TCC)11 repeats with d(GGA),, and d(GGT)11 oligonucleotides, but not with d(TCC)11, gave a mobility-shifted band in poly-acrylamide gel electrophoresis, suggestive of the triplex formation. The two conformers, however, showed difference in binding affinity and structure. The complex formed with d(GGT)11 exhibited patterns protected from DNase I digestion and from modifications with osmium tetroxide and dimethyl sulfate. The patterns were conformed to a proposed triplex model. In contrast, the complex with d(GGA)11 was sensitive to DNase I and to osmium tetroxide, which is reactive to pyrimidine bases of single-stranded DNA. These results suggest that the d(GGA:TCC)11 and d(GGA)11 triple-stranded complex forms a D-loop-like structure. By considering the ability of d(GGA)11 oligonucleotides to form a parallel homoduplex, the structure may be one in which the GGA-strand of the duplex pairs with d(GGA)11 in parallel, with the TCC-strand being looped out. © by the Journal of Biochemistry Regular Papers nationallicence D-loop-like structure nationallicence DNase I footprinting nationallicence osmium tetroxide nationallicence polypurine nationallicence polypyrimidine stretches nationallicence triplex DNA nationallicence Mishima Yukio Department of Biochemistry, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951 aut Suda Takeshi Department of Biochemistry, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951 aut Kominami Ryo Department of Biochemistry, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 805-810 0021-924X 119:4<805 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021311 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021311 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Mishima Yukio Department of Biochemistry, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951 aut NATIONALLICENCE 700 1- Suda Takeshi Department of Biochemistry, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951 aut NATIONALLICENCE 700 1- Kominami Ryo Department of Biochemistry, Niigata University School of Medicine, 1-757 Asahimachi-dori, Niigata 951 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 805-810 0021-924X 119:4<805 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford