caa a22 4500 397557493 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021314 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021314 Isolation, Chemical Characterization, and Subcellular Distribution of 1-O-Alkyl-2-Acetyl-sn-Glycerol in Tetrahymena pyriformis Cells [Elektronische Daten] [Constantinos Tellis, Demokritos C. Tsoukatos, Marilena E. Lekka] 1-O-Hexadecyl-2-acetyl-sn-glycerol, the immediate precursor of platelet-activating factor (PAF) in itsde novo formation,was detected in the protozoon Tetrahymena pyriformis. It was purified from the total lipid extract by TLC, after successive developments in two different solvent systems. Characterization was assessed by (a)gas-liquid chromatography with electron capture detection, and (b)gas chromatography combined with mass spectrometry in selected ion monitoring mode, after derivatization with heptafluorobutyric acid anhydride and teri-butyldimethylchlorosilane/imidazole, respectively. Its quantity was found to be 0.1 nmol/107cells from the GC-MS, using authentic alkylacetylglycerol as external standard. Cell fractionation revealed that alkylacetylglycerol is located exclusively in the microsomal fraction of the protozoon.Previously, we have reported the occurrence of PAF in the microsomal fraction, as well as a dithiothreitol-insensitive CDP-choline: cholinephosphotransferase activity that utilizes exogenous alkylacetylglycerol as substrate in the mitochondrial and microsomal fractions. The above findings indicate that PAF can be formed in the cell by the de novo pathway. © by the Journal of Biochemistry Regular Papers nationallicence alkylacetylglycerol nationallicence diacylglycerols nationallicence GC-MS nationallicence platelet-activating factor nationallicence Tetrahymena pyriformis nationallicence Tellis Constantinos Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece aut Tsoukatos Demokritos C. Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece aut Lekka Marilena E. Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 823-827 0021-924X 119:4<823 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021314 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021314 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Tellis Constantinos Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece aut NATIONALLICENCE 700 1- Tsoukatos Demokritos C. Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece aut NATIONALLICENCE 700 1- Lekka Marilena E. Laboratory of Biochemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 823-827 0021-924X 119:4<823 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford