caa a22 4500 397557531 CHVBK 20180308164807.0 cr unu---uuuuu 161202e199604 xx s 000 0 eng 10.1093/oxfordjournals.jbchem.a021312 doi (NATIONALLICENCE)oxford-10.1093/oxfordjournals.jbchem.a021312 Characterization of a B. subtilis Minor Isoleucine tRNA Deduced from tDNA Having a Methionine Anticodon CAT [Elektronische Daten] [Jitsuhiro Matsugi, Katsutoshi Murao, Hisayuki Ishikura] Bacillus subtilis, which belongs to Gram-positive eubacteria, has been predicted to have a minor isoleucine tRNA transcribed from the gene possessing the CAT anticodon, which corresponds to methionine.We isolated this tRNA and determined its sequence including modified nucleotides. Modified nucleotide analyses using TLC,UV,and FAB mass spectros-copy revealed that the first letter of the anticodon is modified to lysidine [4-amino-2-(N6-lysino)-1-β-D-ribofuranosyl pyrimidine]. As a result, this tRNA agrees with the minor one predicted from the DNA sequence and is thought to decode the isoleucine codon AUA. © by the Journal of Biochemistry Regular Papers nationallicence Bacillus subtilis nationallicence FAB mass spectroscopy nationallicence lysidine nationallicence modified nucleotide nationallicence tRNA nationallicence Matsugi Jitsuhiro Laboratory of Chemistry, Jichi Medical School, 3311-1 Minamikawachi-machi, Tochigi 329-04 aut Murao Katsutoshi Laboratory of Chemistry, Jichi Medical School, 3311-1 Minamikawachi-machi, Tochigi 329-04 aut Ishikura Hisayuki Laboratory of Chemistry, Jichi Medical School, 3311-1 Minamikawachi-machi, Tochigi 329-04 aut The Journal of Biochemistry Oxford University Press 119/4(1996-04), 811-816 0021-924X 119:4<811 1996 119 jbchem https://doi.org/10.1093/oxfordjournals.jbchem.a021312 text/html Onlinezugriff via DOI 1 research-article jats NATIONALLICENCE 856 40 https://doi.org/10.1093/oxfordjournals.jbchem.a021312 text/html Onlinezugriff via DOI NATIONALLICENCE 700 1- Matsugi Jitsuhiro Laboratory of Chemistry, Jichi Medical School, 3311-1 Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Murao Katsutoshi Laboratory of Chemistry, Jichi Medical School, 3311-1 Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 700 1- Ishikura Hisayuki Laboratory of Chemistry, Jichi Medical School, 3311-1 Minamikawachi-machi, Tochigi 329-04 aut NATIONALLICENCE 773 0- The Journal of Biochemistry Oxford University Press 119/4(1996-04), 811-816 0021-924X 119:4<811 1996 119 jbchem Metadata rights reserved CC BY-NC-4.0 http://creativecommons.org/licenses/by-nc/4.0 nationallicence BK010053 XK010053 XK010000 NATIONALLICENCE NATIONALLICENCE NL-oxford